Comparison of chromatographic characteristics of a series of homologous Bence-Jones proteins during size-exclusion chromatography by high-performance liquid chromatography and by Sephadex.
Comparison of chromatographic characteristics of a series of homologous Bence-Jones proteins during size-exclusion chromatography by high-performance liquid chromatography and by Sephadex.
复制标题
通过高效液相色谱和 Sephadex 进行尺寸排阻色谱时一系列同源 Bence-Jones 蛋白的色谱特征比较。
DOI：
10.1016/s0021-9673(01)90406-6
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Stevens,FJ
中科院分区：
文献类型：
--
作者：
Short,MT;Westholm,FA;Schiffer,M;Stevens,FJ
High-performance liquid chromatography has become popular for protein purification and analysis as a consequence of its high resolution capability. Most commonly, protein separations are effected by combined ion exchange and hydrophobic interactions between the protein and the chromatographic matrix. However, the presence of such interaction has impaired the suitability of most porous HPLC matrices for size-exclusion protein fractionation on the basis of molecular weight. Size-exclusion chromatography, which is commonly used for protein purification and molecular weight estimation, has also provided a means to study reversible interactions between proteins. However, analytical interpretation of these experiments requires minimal non-specific interaction between protein and matrix, as is typically the case with polysaccharide or polymerized agarose gels. The authors have previously described the use of small zone gel filtration of immunoglobulin light chains (Bence-Jones proteins) to observe the interactions between these polypeptide chains. Here the authors report the results of similar experiments with these proteins in which they used a commercially available HPLC matrix designed for separation of proteins on the basis of molecular weight.
登录
查看更多内容
本斯-琼斯蛋白和免疫球蛋白轻链（两部分中的第一部分）。
DOI：
--
发表时间：
1976
期刊：
影响因子：
--
作者：
Allan Solomon
通讯作者：
Allan Solomon
TSK-SW 柱上天然蛋白质的高压凝胶渗透色谱。
DOI：
10.1111/j.1399-3011.1981.tb02003.x
发表时间：
2009
期刊：
International journal of peptide and protein research
影响因子：
--
作者：
M. Himmel;P. Squire
通讯作者：
P. Squire
Bence-Jones 蛋白 Au 可变片段二聚化动力学。
DOI：
10.1111/j.1432-1033.1976.tb10866.x
发表时间：
1976
期刊：
European journal of biochemistry
影响因子：
--
作者：
Hiroshi Maeda;Juergen Engel;Hans J. Schramm
通讯作者：
Hans J. Schramm
高效凝胶过滤层析用新型琼脂糖介质的特性
影响因子：
4.1
作者：
L. Hagel;T. Andersson
通讯作者：
T. Andersson
在 Toyopearl HW55F 上对蛋白质进行制备型高速凝胶渗透色谱。
DOI：
10.1016/0006-291x(81)91263-8
发表时间：
1981
影响因子：
3.1
作者：
J. Germershausen;J. D. Karkas
通讯作者：
J. D. Karkas