Qualitative confirmation of 9 synthetic cannabinoids and 20 metabolites in human urine using LC-MS/MS and library search.
Qualitative confirmation of 9 synthetic cannabinoids and 20 metabolites in human urine using LC-MS/MS and library search.
复制标题
DOI：
10.1021/ac3037365
复制
发表时间：
2013-04-02
影响因子：
7.4
通讯作者：
Huestis, Marilyn A.
中科院分区：
文献类型：
--
作者：
Wohlfarth, Ariane;Scheidweiler, Karl B.;Chen, Xiaohong;Liu, Hua-fen;Huestis, Marilyn A.
合成大麻素是一种新兴的非法药物类别。可用物质的种类繁多，而且不断变化，使得实验室很难保持最新状态。我们提出了一种定性LC-MS/MS方法，用于鉴别JWH-018、JWH-073、JWH-081、JWH-122、JWH-200、JWH-210、JWH-250、RCS-4和AM 2201以及母体化合物JWH-018、JWH-073、JWH-081、JWH-122、JWH-210、JWH-250、RCS-4、AM 2201和MAM 2201的尿液代谢物。酶解后，用乙腈沉淀尿蛋白。色谱法使用Kinetex XB-C18柱上的10分钟梯度，使用0.1%甲酸的水溶液和乙腈。在预定的多反应监测“调查扫描”之后，在ABSciex 5500 QTRAP质谱仪上进行信息依赖性采集增强产物离子扫描实验。通过软件辅助库搜索参考光谱鉴别分析物。该方法经过充分验证，包括选择性证明（未观察到外源性或内源性干扰），评估基质效应（95-122%）和回收率（53-95%），测定检测限（0.5-10 ng/mL），残留研究（阈值在100和1000 ng/mL之间），并测定自动进样器稳定性（样品至少稳定3天）。对各种葡萄糖醛酸苷和参比标准品JWH-018 5-羟戊基葡萄糖醛酸苷的水解效率进行了全面研究
Synthetic cannabinoids are an emerging illicit drug class. The variety of available substances is large and ever-changing, making it difficult for laboratories to remain current. We present a qualitative LC–MS/MS method identifying urinary metabolites of JWH-018, JWH-073, JWH-081, JWH-122, JWH-200, JWH-210, JWH-250, RCS-4, and AM2201 and the parent compounds JWH-018, JWH-073, JWH-081, JWH-122, JWH-210, JWH-250, RCS-4, AM2201, and MAM2201. After enzymatic hydrolysis, urinary proteins were precipitated with acetonitrile. Chromatography utilized a 10 min gradient on a Kinetex XB-C18 column with 0.1% formic acid in water and acetonitrile. Scheduled multiple reaction monitoring “survey scans” were followed by information-dependent acquisition-enhanced product ion scan experiments on an ABSciex 5500 QTRAP mass spectrometer. Analytes were identified by software-assisted library searching against reference spectra. The method was fully validated, including proof of selectivity (no exogenous or endogenous interferences were observed), assessment of matrix effects (95–122%) and recovery (53–95%), determination of limits of detection (0.5–10 ng/mL), carry-over studies (thresholds between 100 and 1000 ng/mL), and determination of autosampler stability (samples were stable for at least 3 days). Hydrolysis efficiency was thoroughly investigated for a wide range of glucuronides and for the reference standard, JWH-018 5-hydroxypentyl glucuronide
登录
查看更多内容
影响因子：
2.9
作者：
Moeller, Ines;Wintermeyer, Annette;Thevis, Mario
通讯作者：
Thevis, Mario
影响因子：
2.2
作者：
Gergov, M;Robson, JN;Vuori, E
通讯作者：
Vuori, E
影响因子：
2.5
作者：
Herrin, GL;McCurdy, HH;Wall, WH
通讯作者：
Wall, WH
影响因子：
2.5
作者：
Kavanagh, Pierce;Grigoryev, Andrej;Simonov, Anton
通讯作者：
Simonov, Anton
影响因子：
4.3
作者：
Wintermeyer, Annette;Moeller, Ines;Bender, Katja
通讯作者：
Bender, Katja