Inhibition of Apoplastic Calmodulin Impairs Calcium Homeostasis and Cell Wall Modeling during Cedrus deodara Pollen Tube Growth
Inhibition of Apoplastic Calmodulin Impairs Calcium Homeostasis and Cell Wall Modeling during Cedrus deodara Pollen Tube Growth
复制标题
质外体钙调蛋白的抑制会损害雪松花粉管生长过程中的钙稳态和细胞壁建模
DOI：
10.1371/journal.pone.0055411
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发表时间：
2013-02
期刊：
影响因子：
3.7
通讯作者：
T Chen
中科院分区：
文献类型：
--
作者：
M Zhang;H Wang;B Jin;T Chen
钙调素（CaM）是真核细胞中研究最多的钙离子转导蛋白之一。它是已知的调节许多蛋白质的活性与不同的细胞功能，然而，质外体钙调素在植物细胞中的功能仍然知之甚少。采用药理分析和显微技术相结合的方法，研究了雪松（Cedrus deodara（Roxb.）大声了结果发现，尖端聚焦的钙梯度被迅速扰乱后，应用药物的早期事件之一，而细胞质的组织没有受到显着影响。酸性果胶和酯化果胶的沉积和分布也发生了显着变化，进一步扰乱了细胞壁的正常建模。来自不同功能类别的几种蛋白候选物可能参与对质外体CaM抑制的反应。这些结果表明，质外体钙调素的功能，以维持尖端集中的钙梯度和调节果胶的分布/转化过程中的花粉管生长。
Calmodulin (CaM) is one of the most well-studied Ca2+ transducers in eukaryotic cells. It is known to regulate the activity of numerous proteins with diverse cellular functions; however, the functions of apoplastic CaM in plant cells are still poorly understood. By combining pharmacological analysis and microscopic techniques, we investigated the involvement of apoplastic CaM in pollen tube growth of Cedrus deodara (Roxb.) Loud. It was found that the tip-focused calcium gradient was rapidly disturbed as one of the early events after application of pharmacological agents, while the cytoplasmic organization was not significantly affected. The deposition and distribution of acidic pectins and esterified pectins were also dramatically changed, further perturbing the normal modeling of the cell wall. Several protein candidates from different functional categories may be involved in the responses to inhibition of apoplastic CaM. These results revealed that apoplastic CaM functions to maintain the tip-focused calcium gradient and to modulate the distribution/transformation of pectins during pollen tube growth.
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影响因子：
4.1
作者：
CROCKER, G;DAWSON, RA;MACNEIL, S
通讯作者：
MACNEIL, S
影响因子：
4.3
作者：
J. Zeier;L. Schreiber
通讯作者：
J. Zeier;L. Schreiber
影响因子：
4.8
作者：
Cui, SJ;Guo, XQ;Sun, D
通讯作者：
Sun, D
影响因子：
4
作者：
M. McCann;N. Stacey;R. Wilson;K. Roberts
通讯作者：
M. McCann;N. Stacey;R. Wilson;K. Roberts
影响因子：
4.3
作者：
E. Yokota;T. Ohmori;S. Muto;T. Shimmen
通讯作者：
E. Yokota;T. Ohmori;S. Muto;T. Shimmen