Hydrodynamics in Cell Studies.
Hydrodynamics in Cell Studies.
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DOI：
10.1021/acs.chemrev.7b00317
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发表时间：
2018-02-28
期刊：
影响因子：
62.1
通讯作者：
Kaigala GV
中科院分区：
文献类型：
--
作者：
Huber D;Oskooei A;Casadevall I Solvas X;Andrew deMello;Kaigala GV
流体动力学现象在生物体中普遍存在，可用于操纵细胞或模拟体内经历的生理微环境。水动力效应影响多种细胞特性和过程，包括细胞形态、细胞内过程、细胞-细胞信号级联和反应动力学，并在单细胞、多细胞和器官水平上发挥重要作用。选定的流体动力效应也可以用来控制细胞微环境中的机械应力、分析物运输以及局部温度。随着对微米尺度流体力学的更好理解和微流体技术的出现，新一代实验工具可以控制细胞微环境，并以精确的精度模拟生理条件，现在正在出现。因此，我们认为现在是时候评估细胞微环境的水动力控制及其应用的概念，并对这些工具在体外细胞培养模型中的未来提供一些观点。一般来说，我们描述了活细胞、流体动力压力源和施加在细胞上的流体流动诱导效应之间的相互作用。这种相互作用导致了细胞内部和周围广泛的化学、生物和物理现象。更具体地说，我们描述和制定影响粘附和悬浮细胞的水动力现象的基本物理。此外，我们还概述了在微流体系统中单细胞研究背景下利用流体动力学效应的代表性研究。
Hydrodynamic phenomena are ubiquitous in living organisms and can be used to manipulate cells or emulate physiological microenvironments experienced in vivo. Hydrodynamic effects influence multiple cellular properties and processes, including cell morphology, intracellular processes, cell–cell signaling cascades and reaction kinetics, and play an important role at the single-cell, multicellular, and organ level. Selected hydrodynamic effects can also be leveraged to control mechanical stresses, analyte transport, as well as local temperature within cellular microenvironments. With a better understanding of fluid mechanics at the micrometer-length scale and the advent of microfluidic technologies, a new generation of experimental tools that provide control over cellular microenvironments and emulate physiological conditions with exquisite accuracy is now emerging. Accordingly, we believe that it is timely to assess the concepts underlying hydrodynamic control of cellular microenvironments and their applications and provide some perspective on the future of such tools in in vitro cell-culture models. Generally, we describe the interplay between living cells, hydrodynamic stressors, and fluid flow-induced effects imposed on the cells. This interplay results in a broad range of chemical, biological, and physical phenomena in and around cells. More specifically, we describe and formulate the underlying physics of hydrodynamic phenomena affecting both adhered and suspended cells. Moreover, we provide an overview of representative studies that leverage hydrodynamic effects in the context of single-cell studies within microfluidic systems.
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DOI：
10.1016/j.snb.2013.10.002
发表时间：
2014-03-01
期刊：
Sensors and actuators. B, Chemical
影响因子：
--
作者：
Bell L;Seshia A;Lando D;Laue E;Palayret M;Lee SF;Klenerman D
通讯作者：
Klenerman D
影响因子：
4
作者：
Bodnar, T.;Sequeira, A.;Prosi, M.
通讯作者：
Prosi, M.
影响因子：
1.6
作者：
BONNER, WA;SWEET, RG;HERZENBERG, LA
通讯作者：
HERZENBERG, LA
影响因子：
2.4
作者：
Bouffanais, Roland;Sun, Jianmin;Yue, Dick K. P.
通讯作者：
Yue, Dick K. P.
影响因子：
64.8
作者：
ALON, R;HAMMER, DA;SPRINGER, TA
通讯作者：
SPRINGER, TA