A tubular endosomal fraction from rat liver: biochemical evidence of receptor sorting by default.
A tubular endosomal fraction from rat liver: biochemical evidence of receptor sorting by default.
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来自大鼠肝脏的管状内体部分：默认情况下受体分选的生化证据。
DOI：
10.1073/pnas.96.18.10146
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发表时间：
1999
影响因子：
11.1
通讯作者：
Mostov,KE
中科院分区：
文献类型：
--
作者：
Vergés,M;Havel,RJ;Mostov,KE
我们之前已经从大鼠肝脏中分离出一种内体组分，称为受体再循环室(RRC)，它高度富含循环受体和跨细胞聚合免疫球蛋白受体(PIgR)。我们现在已经通过免疫分离分析了RRC组分，发现不存在唯一的跨细胞元件，因为循环受体和pIgR是在相同的元件上共分离的。此外，RRC富含以前被证明与内小体循环相关的蛋白质，如Rab 11、cellubrevin和endobrevin，但相对较少的是早期内体抗原1。由于RRC主要包含小管和小囊泡，我们的结果表明它富含参与受体分选的管状内体隔室的元件。生化分析表明，RRC膜的循环受体密度和跨细胞pIgR密度与早期内吞体膜相似。这一观察结果支持这样的观点，即通过内吞体管作用增加膜表面积是确保有效受体分选的主要机制，同时，在最终受体分离到不同的循环和跨细胞途径之前，RRC位于内吞系统的共同步骤。
We previously have isolated an endosomal fraction from rat liver, termed receptor-recycling compartment (RRC), which is highly enriched in recycling receptors and in the transcytotic polymeric Ig receptor (pIgR). We now have analyzed the RRC fraction by immunoisolation and found that no uniquely transcytotic elements were present, because recycling receptors and the pIgR were coisolated on the same elements. In addition, RRC was very rich in proteins previously shown to be associated with recycling endosomes, such as rab 11, cellubrevin, and endobrevin, but relatively poor in early endosome antigen 1. As RRC contains mainly tubules and small vesicles, our results indicate that it is enriched in elements of a tubular endosomal compartment involved in receptor sorting. Biochemical analysis showed that the density of recycling receptors and transcytotic pIgR in RRC membranes was similar to that in early endosome membranes. This observation supports the idea that increasing membrane surface area by endosome tubulation is the main mechanism to ensure efficient receptor sorting and, at the same time, locates RRC in a common step of the endocytotic system before final receptor segregation into distinct recycling and transcytotic pathways.
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影响因子：
7
作者：
P. Pierre;I. Mellman
通讯作者：
P. Pierre;I. Mellman
影响因子：
3.3
作者：
Casanova, JE;Wang, XY;Goldenring, JR
通讯作者：
Goldenring, JR
用 17 α-乙炔雌二醇处理的大鼠和人类脂蛋白的肝脏分解代谢。
影响因子：
4.8
作者：
Y. Chao;E. Windler;G. Chen;R. Havel
通讯作者：
R. Havel
大鼠肝脏分离内体部分中蛋白质的鉴定和分布：参与内吞作用、再循环和转胞吞作用。
影响因子：
4.1
作者：
A. Pol;David Ortega;C. Enrich
通讯作者：
C. Enrich
DOI：
10.1083/jcb.141.3.611
发表时间：
1998-05-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Futter CE;Gibson A;Allchin EH;Maxwell S;Ruddock LJ;Odorizzi G;Domingo D;Trowbridge IS;Hopkins CR
通讯作者：
Hopkins CR