Investigation of the association between clinical outcome and the cag pathogenicity-island and other virulence genes of Helicobacter pylori isolates from patients with dyspepsia in Eastern Turkey
Investigation of the association between clinical outcome and the cag pathogenicity-island and other virulence genes of Helicobacter pylori isolates from patients with dyspepsia in Eastern Turkey
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土耳其东部消化不良患者幽门螺杆菌分离株 cag 致病岛及其他毒力基因与临床结果之间关系的调查
DOI：
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发表时间：
2013
影响因子：
2.2
通讯作者：
H. Ertaş
中科院分区：
文献类型：
--
作者：
G. Ozbey;U. Demirel;C. Aygun;H. Ertaş
我们的工作目的是确定胃炎和消化性溃疡患者恢复的幽门螺杆菌cag致病性岛（cag PAI）和其他毒力基因的存在，并探讨这些毒力基因与临床预后的相关性。采用聚合酶链反应法测定69株幽门螺杆菌cagA、cagA启动子区、cagE、cagT启动子区、cag- pai左端启动子区（LEC）、cag右连接区（cagRJ）、开放阅读框（ORFs）、vacA和oipA基因的存在。完整的cag PAI仅在1株（1.4%）分离物中检测到。cagA基因在消化不良（胃炎和消化性溃疡）患者分离株中分别占52.1%和76.2%。在消化性溃疡分离菌中主要检测到可塑性区orf，即JHP912和JHP931。不到25%的分离株携带其他orf。菌株中以ⅰ型、ⅱ型和ⅲ型最为常见。没有分离株具有Ib、1c、IIIb、IV和V型基序。在消化性溃疡和胃炎分离株中，最常见的vacA基因型分别为s1am1a和s1m2。结果证实，oipA （Hp0638）基因在胃炎和消化性溃疡患者中的患病率分别为75%和85.7%。此外，vacA s1am1a阳性与消化性溃疡相关（p < 0.05）。
The aims of our work were to determine the presence of the cag pathogenicity-island (cag PAI) and other virulence genes of Helicobacter pylori recovered from patients with gastritis and peptic ulcer, and to investigate the correlation of these virulence genes with clinical outcome. The presence of the cagA, the promoter regions of cagA, cagE, cagT, and the left end of cag-PAI (LEC), cag right junction (cagRJ), the plasticity region open reading frames (ORFs), vacA and oipA genes among 69 H. pylori isolates were determined by polymerase chain reaction. Intact cag PAI was detected in only one (1.4%) isolate. The cagA gene was identified in 52.1% and 76.2% of isolates from patients with dyspepsia (gastritis and peptic ulcer), respectively. The plasticity region ORFs i.e. JHP912 and JHP931 were predominantly detected in isolates from peptic ulcer. Less than 25% of the isolates carried other ORFs. Types I, II and III were the most commonly found among the isolates. None of the isolates possessed type Ib, 1c, IIIb, IV and V motifs. The most commonly vacA genotypes were s1am1a and s1m2 in isolates with peptic ulcer and gastritis, respectively. The results confirmed that the prevalence of oipA (Hp0638) gene was 75% and 85.7% in patients with gastritis and peptic ulcer, respectively. Furthermore, vacA s1am1a positivity was significantly related to peptic ulcer (p < 0.05).
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影响因子：
11.2
作者：
M. Blaser;G. Perez-Perez-G.-Perez-Perez-82101131;H. Kleanthous;T. Cover;R. Peek;P. Chyou;G. Stemmermann;A. Nomura
通讯作者：
M. Blaser;G. Perez-Perez-G.-Perez-Perez-82101131;H. Kleanthous;T. Cover;R. Peek;P. Chyou;G. Stemmermann;A. Nomura
DOI：
10.1016/j.dld.2009.01.010
发表时间：
2009-09
期刊：
Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver
影响因子：
--
作者：
Baghaei K;Shokrzadeh L;Jafari F;Dabiri H;Yamaoka Y;Bolfion M;Zojaji H;Aslani MM;Zali MR
通讯作者：
Zali MR
DOI：
10.1007/978-1-62703-005-2_7
发表时间：
2012
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
通讯作者：
--
影响因子：
29.4
作者：
Yamaoka, Y;Kikuchi, S;Graham, DY
通讯作者：
Graham, DY