Ste50 adaptor protein governs sexual differentiation of Cryptococcus neoformans via the pheromone-response MAPK signaling pathway.
Ste50 adaptor protein governs sexual differentiation of Cryptococcus neoformans via the pheromone-response MAPK signaling pathway.
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DOI：
10.1016/j.fgb.2010.10.006
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发表时间：
2011-02
影响因子：
3
通讯作者：
Bahn, Yong-Sun
中科院分区：
文献类型：
--
作者：
Jung, Kwang-Woo;Kim, Seo-Young;Okagaki, Laura H.;Nielsen, Kirsten;Bahn, Yong-Sun
丝裂原活化蛋白激酶（MAPK）途径控制病原真菌的多种细胞功能，包括性分化、应激反应和维持细胞壁完整性。在这里，我们描述了一个C。neoformans基因，其与酵母Ste 50同源，已知其作为酿酒酵母中Ste 11 MAPK激酶激酶的衔接蛋白在交配信息素应答和应激应答中发挥重要作用。梭新形虫Ste 50不参与由HOG和Ras/cAMP信号传导途径控制的任何应激反应或毒力因子产生（荚膜和黑色素）。然而，在血清型A和血清型D C中，交配都需要Ste 50。neoformans菌株。ste 50 Δ突变体在细胞融合和交配信息素产生方面完全缺陷。STE 50基因的双突变阻断了CRG 1基因缺失的细胞的信息素产生增加和超表达表型，CRG 1基因编码RGS蛋白，其通过MAPK途径负调节信息素应答性G蛋白信号传导。尽管存在已知玉米黑粉菌完全毒力所需的Ste 50的担子菌特异性SH 3结构域，但Ste 50对玉米黑粉菌的毒力是不确定的。隐球菌病小鼠模型中的新型球菌。综上所述，Ste 50衔接蛋白控制着C.通过信息素应答的MAPK途径而不是毒力所需的。
The mitogen-activated protein kinase (MAPK) pathways control diverse cellular functions in pathogenic fungi, including sexual differentiation, stress-response, and maintenance of cell wall integrity. Here we characterized a C. neoformans gene, which is homologous to the yeast Ste50 that is known to play an important role in mating pheromone response and stress response as an adaptor protein to the Ste11 MAPK kinase kinase in Saccharomyces cerevisiae. The C. neoformans Ste50 was not involved in any of the stress responses or virulence factor production (capsule and melanin) that are controlled by the HOG and Ras/cAMP signaling pathways. However, Ste50 was required for mating in both serotype A and serotype D C. neoformans strains. The ste50Δ mutant was completely defective in cell-cell fusion and mating pheromone production. Double mutation of the STE50 gene blocked increased production of pheromone and the hyper-filamentation phenotype of cells deleted of the CRG1 gene, which encodes the RGS protein that negatively regulates pheromone responsive G-protein signaling via the MAPK pathway. Regardless of the presence of the basidiomycota-specific SH3 domains of Ste50 that are known to be required for full virulence of Ustilago maydis, Ste50 was dispensable for virulence of C. neoformans in a murine model of cryptococcosis. In conclusion, the Ste50 adaptor protein controls sexual differentiation of C. neoformans via the pheromone-responsive MAPK pathway but is not required for virulence.
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