Use of a density modification technique for isolation of the plasma membrane of rod outer segments.
Use of a density modification technique for isolation of the plasma membrane of rod outer segments.
复制标题
使用密度修改技术来分离棒外节的质膜。
DOI：
10.1016/0005-2736(82)90558-2
复制
发表时间：
1982
期刊：
影响因子：
--
通讯作者：
F. Daemen
中科院分区：
文献类型：
--
作者：
K. Kamps;W. D. de Grip;F. Daemen
The external surface of rod outer segments contains receptors for the Jack Bean lectin, concanavalin A. Using isolated intact bovine rod outer segments, we have modified the density of the outer segment plasma membranes by means of polystyrene beads carrying covalently linked concanavalin A. After hypotonic lysis the bulk of the disk membranes can be removed and a plasma membrane fraction is isolated. The plasma membrane preparation contains 1.5% of total outer segment rhodopsin and 2.7% of total outer segment protein. It shows very little contamination with inner segment plasma membrane. Contamination with disk membranes appears to be low as well. Fatty acid analysis reveals that the plasma membranes are more saturated than the highly unsaturated disk membranes. Gel electrophoresis shows the presence of at least six additional polypeptides besides rhodopsin.
影响因子：
6.5
作者：
W. R. Morrison;L. Smith
通讯作者：
W. R. Morrison;L. Smith