Expression profile of microRNAs regulating proliferation and differentiation in mouse adult cardiac stem cells.
Expression profile of microRNAs regulating proliferation and differentiation in mouse adult cardiac stem cells.
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DOI：
10.1371/journal.pone.0063041
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Gama-Carvalho M
中科院分区：
文献类型：
--
作者：
Brás-Rosário L;Matsuda A;Pinheiro AI;Gardner R;Lopes T;Amaral A;Gama-Carvalho M
具有干细胞特性的心脏细胞的鉴定改变了心脏作为有丝分裂后器官的范式。这些细胞增殖并分化为心肌细胞、血管内皮细胞和血管平滑肌细胞，提供心肌细胞的动态平衡和再生。MicroRNAs是控制增殖和分化的主开关，特别是调节干细胞生物学和心脏发育。调控microRNAs调控的基因表达网络具有控制细胞命运和增殖的潜力，具有可预测的生物技术和治疗应用。为了深入了解心脏干细胞中活跃的调控网络，我们描述了95个具有干细胞和组织分化功能的microRNAs在小鼠心脏干细胞中的表达谱，并将其与小鼠胚胎心脏和间充质干细胞的表达谱进行了比较。在心脏干细胞中发现的表达最高的microRNAs是已知的靶基因，涉及控制细胞增殖和黏附、血管功能和心肌细胞分化的关键基因。我们报道了一组差异表达的microRNAs，它们被认为是成年心脏干细胞分化和增殖的调节因子，为调节其生物学特性的活性基因表达网络提供了新的见解。
The identification of cardiac cells with stem cell properties changed the paradigm of the heart as a post mitotic organ. These cells proliferate and differentiate into cardiomyocytes, endothelial and vascular smooth muscle cells, providing for cardiac cell homeostasis and regeneration. microRNAs are master switches controlling proliferation and differentiation, in particular regulating stem cell biology and cardiac development. Modulation of microRNAs -regulated gene expression networks holds the potential to control cell fate and proliferation, with predictable biotechnologic and therapeutic applications. To obtain insights into the regulatory networks active in cardiac stem cells, we characterized the expression profile of 95 microRNAs with reported functions in stem cell and tissue differentiation in mouse cardiac stem cells, and compared it to that of mouse embryonic heart and mesenchymal stem cells. The most highly expressed microRNAs identified in cardiac stem cells are known to target key genes involved in the control of cell proliferation and adhesion, vascular function and cardiomyocyte differentiation. We report a subset of differentially expressed microRNAs that are proposed to act as regulators of differentiation and proliferation of adult cardiac stem cells, providing novel insights into active gene expression networks regulating their biological properties.
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