Simultaneous Infection With Porcine Reproductive and Respiratory Syndrome and Influenza Viruses Abrogates Clinical Protection Induced by Live Attenuated Porcine Reproductive and Respiratory Syndrome Vaccination.
Simultaneous Infection With Porcine Reproductive and Respiratory Syndrome and Influenza Viruses Abrogates Clinical Protection Induced by Live Attenuated Porcine Reproductive and Respiratory Syndrome Vaccination.
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DOI：
10.3389/fimmu.2021.758368
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发表时间：
2021
影响因子：
7.3
通讯作者：
Tchilian E
中科院分区：
文献类型：
--
作者：
Chrun T;Maze EA;Vatzia E;Martini V;Paudyal B;Edmans MD;McNee A;Manjegowda T;Salguero FJ;Wanasen N;Koonpaew S;Graham SP;Tchilian E
猪呼吸道疾病复合体(PRDC)是造成全球养猪业重大经济损失的主要原因。猪繁殖与呼吸综合征病毒(PRRSV)和猪流感病毒是引起PRDC的主要病毒因子。疫苗是控制PRRS的具有成本效益的措施，然而，它们在合并感染背景下的有效性一直没有得到很好的调查。本研究旨在探讨PRRSV-2与猪流感H3N2病毒混合感染对广泛应用的PRRSV修饰活病毒(MLV)疫苗免疫效果的影响。在同时攻击PRRSV-2和H3N1野毒株后，我们发现PRRS MLV疫苗接种对临床疾病和病理的保护作用被取消，尽管病毒载量没有受到影响，抗体应答得到增强。相比之下，未免疫动物的混合感染降低了上呼吸道的PRRSV-2病毒血症和H3N2病毒载量，并增强了肺部对PRRSV-2和H3N2T细胞的反应。进一步的分析表明，免疫猪肺部抑制细胞因子基因表达的上调可能影响了对H3N2和PRRSV-2的反应。这些发现为病毒混合感染对PRRS疫苗效力的影响提供了重要的见解，可能有助于在现场确定更有效的针对PRDC的疫苗接种策略。
The porcine respiratory disease complex (PRDC) is responsible for significant economic losses in the pig industry worldwide. Porcine reproductive and respiratory syndrome virus (PRRSV) and swine influenza virus are major viral contributors to PRDC. Vaccines are cost-effective measures for controlling PRRS, however, their efficacy in the context of co-infections has been poorly investigated. In this study, we aimed to determine the effect of PRRSV-2 and swine influenza H3N2 virus co-infection on the efficacy of PRRSV modified live virus (MLV) vaccination, which is widely used in the field. Following simultaneous challenge with contemporary PRRSV-2 and H3N2 field isolates, we found that the protective effect of PRRS MLV vaccination on clinical disease and pathology was abrogated, although viral load was unaffected and antibody responses were enhanced. In contrast, co-infection in non-immunized animals reduced PRRSV-2 viremia and H3N2 virus load in the upper respiratory tract and potentiated T cell responses against both PRRSV-2 and H3N2 in the lung. Further analysis suggested that an upregulation of inhibitory cytokines gene expression in the lungs of vaccinated pigs may have influenced responses to H3N2 and PRRSV-2. These findings provide important insights into the effect of viral co-infections on PRRS vaccine efficacy that may help identify more effective vaccination strategies against PRDC in the field.
影响因子：
2.6
作者：
Sattler T;Pikalo J;Wodak E;Revilla-Fernández S;Steinrigl A;Bagó Z;Entenfellner F;Claude JB;Pez F;Francillette M;Schmoll F
通讯作者：
Schmoll F