Light and electron microscopic analysis of tattoos treated by Q-switched ruby laser.

Light and electron microscopic analysis of tattoos treated by Q-switched ruby laser.
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Q 开​​关红宝石激光治疗纹身的光和电子显微镜分析。

DOI:
10.1111/1523-1747.ep12478570
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发表时间:
1991
期刊:
The Journal of investigative dermatology
影响因子:
--
通讯作者:
Flotte,TJ
Flotte,TJ
中科院分区:
--
文献类型:
--
作者:
Taylor,CR;Anderson,RR;Gange,RW;Michaud,NA;Flotte,TJ

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短脉冲激光照射可用于通过选择性光热作用改变组织中的色素结构。通过光镜和电镜观察,探讨了Q开关红宝石激光对人体文身色素增亮的可能机制。纹身之间和纹身内部存在显著差异。未处理的纹身的电子显微镜显示膜结合的色素颗粒,主要是在成纤维细胞和巨噬细胞,偶尔在肥大细胞。这些颗粒含有直径为2英寸的颜料颗粒。暴露后立即在含有色素的细胞中观察到剂量相关损伤。有些色素颗粒较小,呈片状。在能量密度≥ 3 J/cm 2时,偶见真皮空泡和紧邻细胞外色素的胶原束均质化。到24 h时,明显可见活跃的嗜中性粒细胞浸润。11天后,色素再次出现在细胞内。在150 d时,一半的活检显示轻度持续性淋巴细胞浸润。除1例临床瘢痕形成外,无纤维化。这些发现证实,短脉冲辐射可用于选择性破坏含有纹身色素的细胞。色素颗粒的物理改变、再分布和消除似乎是纹身临床变浅的原因。
Short-pulse laser exposures can be used to alter pigmented structures in tissue by selective photothermolysis. Potential mechanisms of human tattoo pigment lightening with Q-switched ruby laser were explored by light and electron microscopy. Significant variation existed between and within tattoos. Electron microscopy of untreated tattoos revealed membrane-bound pigment granules, predominantly within fibroblasts and macrophages, and occasionally in mast cells. These granules contained pigment particles ranging from 2-in diameter. Immediately after exposure, dose-related injury was observed in cells containing pigment. Some pigment particles were smaller and lamellated. At fluences ≥ 3 J/cm2, dermal vacuoles and homogenization of collagen bundles immediately adjacent to extracellular pigment were occasionally observed. A brisk neutrophilic infiltrate was apparent by 24 h. Eleven days later, the pigment was again intracellular. Half of the biopsies at 150 d revealed a mild persistent lymphocytic infiltrate. There was no fibrosis except for one case of clinical scarring. These findings confirm that short-pulse radiation can be used to selectively disrupt cells containing tattoo pigments. The physical alteration of pigment granules, redistribution, and elimination appear to account for clinical lightening of the tattoos.
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