ParA's Impact beyond Chromosome Segregation in Caulobacter crescentus.
ParA's Impact beyond Chromosome Segregation in Caulobacter crescentus.
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DOI：
10.1128/jb.00296-22
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发表时间：
2023-02-22
影响因子：
3.2
通讯作者：
Mera, Paola E.
中科院分区：
文献类型：
--
作者：
Menikpurage, Inoka P.;Puentes-Rodriguez, Stephanie G.;Elaksher, Rawan A.;Mera, Paola E.
在每个细胞周期完成后保持正确的染色体遗传对细菌的生存至关重要。关于细菌如何在每个细胞周期后保留完整染色体的机制细节仍然不完整。在这项研究中，我们研究了分配蛋白帕拉对染色体维护的潜在作用，超越了引发新月柄杆菌染色体分离的发生。我们的数据显示，增加帕拉的水平导致在细胞中的多个复制起点在DnaA-ATP依赖的方式。即使当表达在促进染色体分离方面有缺陷的帕拉变异体时，这种ori额外的保留。我们的数据表明，在柄杆菌帕拉的影响复制启动可能是间接的，可能通过其他细胞周期事件的影响。总的来说，我们的数据为推动细菌细胞周期向前发展的高度互联网络提供了新的见解。重要性成功产生含有完整染色体拷贝的子细胞需要主要细胞周期事件的精密协调。这种协调中的任何错误都可能是致命的，使这些过程成为新型抗生素的理想目标。在这项研究中，我们专注于染色体复制的发病之间的协调，和分区蛋白ParA。我们表明，改变细胞水平的帕拉导致细胞积累多个复制源新月柄杆菌。我们的工作提供了重要的见解复杂的调控参与协调的细菌细胞周期。
Maintaining proper chromosome inheritance after the completion of each cell cycle is paramount for bacterial survival. Mechanistic details remain incomplete for how bacteria manage to retain complete chromosomes after each cell cycle. In this study, we examined the potential roles of the partitioning protein ParA on chromosomal maintenance that go beyond triggering the onset of chromosome segregation in Caulobacter crescentus. Our data revealed that increasing the levels of ParA result in cells with multiple origins of replication in a DnaA-ATP-dependent manner. This ori supernumerary is retained even when expressing variants of ParA that are deficient in promoting chromosome segregation. Our data suggest that in Caulobacter ParA’s impact on replication initiation is likely indirect, possibly through the effect of other cell cycle events. Overall, our data provide new insights into the highly interconnected network that drives the forward progression of the bacterial cell cycle. IMPORTANCE The successful generation of a daughter cell containing a complete copy of the chromosome requires the exquisite coordination of major cell cycle events. Any mistake in this coordination can be lethal, making these processes ideal targets for novel antibiotics. In this study, we focused on the coordination between the onset of chromosome replication, and the partitioning protein ParA. We demonstrate that altering the cellular levels of ParA causes cells to accumulate multiple origins of replication in Caulobacter crescentus. Our work provides important insights into the complex regulation involved in the coordination of the bacterial cell cycle.
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影响因子：
9.2
作者：
Jonas, Kristina;Chen, Y. Erin;Laub, Michael T.
通讯作者：
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影响因子：
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作者：
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通讯作者：
Schumacher, Maria A.
影响因子：
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作者：
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通讯作者：
Thanbichler, Martin
影响因子：
3.7
作者：
Iniesta AA
通讯作者：
Iniesta AA