Beyond Antibodies as Binding Partners: The Role of Antibody Mimetics in Bioanalysis.
Beyond Antibodies as Binding Partners: The Role of Antibody Mimetics in Bioanalysis.
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DOI：
10.1146/annurev-anchem-061516-045205
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发表时间：
2017-06-12
期刊：
影响因子：
--
通讯作者：
Daunert S
中科院分区：
文献类型：
--
作者：
Yu X;Yang YP;Dikici E;Deo SK;Daunert S
能够以类似于抗原-抗体相互作用的方式与配体分析物结合的新型结合蛋白或抗体模拟物的出现已经刺激了生物技术和生物分析界的兴趣增加。目标是生产设计为在结合亲和力、细胞和肿瘤渗透、大规模生产以及温度和pH稳定性方面优于抗体的抗体模拟物。具有定制特征的抗体模拟物的产生涉及鉴定天然存在的蛋白质支架作为与所需配体结合的模板。然后通过首先产生文库，然后使文库经历一系列选择步骤，将该支架工程化以产生上级结合剂。抗体模拟物已成功地用于开发用于检测生物样品中分析物的结合测定，以及用于分离方法、癌症治疗、靶向药物递送和体内成像。本文综述了抗体模拟物及其在生物分析化学，特别是在诊断和其他分析方法中的应用。
The emergence of novel binding proteins or antibody mimetics capable of binding to ligand analytes in a manner analogous to that of the antigen–antibody interaction has spurred increased interest in the biotechnology and bioanalytical communities. The goal is to produce antibody mimetics designed to outperform antibodies with regard to binding affinities, cellular and tumor penetration, large-scale production, and temperature and pH stability. The generation of antibody mimetics with tailored characteristics involves the identification of a naturally occurring protein scaffold as a template that binds to a desired ligand. This scaffold is then engineered to create a superior binder by first creating a library that is then subjected to a series of selection steps. Antibody mimetics have been successfully used in the development of binding assays for the detection of analytes in biological samples, as well as in separation methods, cancer therapy, targeted drug delivery, and in vivo imaging. This review describes recent advances in the field of antibody mimetics and their applications in bioanalytical chemistry, specifically in diagnostics and other analytical methods.
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影响因子：
16.6
作者：
Desmet, Johan;Verstraete, Kenneth;Bloch, Yehudi;Lorent, Eric;Wen, Yurong;Devreese, Bart;Vandenbroucke, Karen;Loverix, Stefan;Hettmann, Thore;Deroo, Sabrina;Somers, Klaartje;Henderikx, Paula;Lasters, Ignace;Savvides, Savvas N.
通讯作者：
Savvides, Savvas N.
影响因子：
5.6
作者：
Eggel, Alexander;Baumann, Michael J.;Vogel, Monique
通讯作者：
Vogel, Monique
DOI：
10.1002/anie.201603488
发表时间：
2016-08-16
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Cox N;Kintzing JR;Smith M;Grant GA;Cochran JR
通讯作者：
Cochran JR
影响因子：
--
作者：
Casini N;Forte IM;Mastrogiovanni G;Pentimalli F;Angelucci A;Festuccia C;Tomei V;Ceccherini E;Di Marzo D;Schenone S;Botta M;Giordano A;Indovina P
通讯作者：
Indovina P
影响因子：
5.6
作者：
DICKINSON, CD;VEERAPANDIAN, B;ELY, KR
通讯作者：
ELY, KR