Detection of PCV2e strains in Southeast China.
Detection of PCV2e strains in Southeast China.
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作者：
Liu J;Wei C;Dai A;Lin Z;Fan K;Fan J;Liu J;Luo M;Yang X
猪圆环病毒2型（Porcine Circovirus 2，PCV 2）自2002年以来在我国猪群中流行，给养猪业造成了严重的经济损失。中国东南部生猪存栏量> 2000万头。由于福建省PCV 2遗传变异的信息有限，本工作的目的是调查2013年至2017年中国东南部PCV 2的流行病学和进化特征。在2013年至2017年从90个不同猪群收集的685份样本中，来自84个不同猪群的356份样本对PCV 2呈阳性。福建省猪群中PCV 2a、PCV 2b、PCV 2d和PCV 2 e共存，其中PCV 2d为主要流行株，PCV 2 e为国内首次报道。引人注目的是，PCV 2-FJ-水DNA来自受污染的河水，而不是受感染的动物。结果表明，95个分离物的开放阅读框2（ORF 2）核苷酸同源性为78.7%~ 100%，氨基酸同源性为74.5%~ 100%。氨基酸序列比对结果表明，PCV 2 e的Cap蛋白与PCV 2a、PCV 2b、PCV 2c和PCV 2d的Cap蛋白存在显著差异。这些结果表明，PCV 2在中国存在不同的基因型，并且PCV 2在不断进化，导致新的变异株迅速出现。
Porcine circovirus 2 (PCV2) has been prevalent in swine herds in China since 2002, causing severe economic loss to the pig industry. The number of live pigs in southeast China is > 20 million. Since information on the genetic variation of PCV2 in the Fujian province is limited, the objective of the present work was to investigate the epidemiological and evolutionary characteristics of PCV2 in southeast China from 2013 to 2017. Of the 685 samples collected from 90 different swine herds from 2013 to 2017, 356 samples from 84 different swine herds were positive for PCV2. PCV2a, PCV2b, PCV2d, and PCV2e co-existed in the Fujian province, with PCV2d being the predominant circulating strain in swineherds and PCV2e being reported for the first time in China. Strikingly, PCV2-FJ-water DNA comes from contaminated river water and not infected animals. Sequence comparison among all isolates indicated that 95 isolates shared approximately 78.7%–100% nucleotide identity and 74.5%–100% amino acid identity for open reading frame 2 (ORF2). Amino acid alignment showed that the Cap protein of PCV2e differed markedly from those of PCV2a, PCV2b, PCV2c, and PCV2d. These results indicated that various PCV2 genotypes exist in China, and that PCV2 is continuously evolving, leading to rapid emergence of new variant stains.
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