Molecular characterization of an elasmobranch urea transporter.
Molecular characterization of an elasmobranch urea transporter.
复制标题
软骨鱼尿素转运蛋白的分子表征。
DOI：
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
P. Wright
中科院分区：
文献类型：
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作者：
Craig P. Smith;P. Wright
海洋板鳃鱼保持相对较高的尿素水平，以平衡生活在海水中的渗透压力。为了维持渗透平衡和降低制造尿素的能量成本，这些动物将尿素排泄到环境中是很重要的。我们从角鲨（Squalus acanthias）的肾脏中分离出一个新的2.2 kb cDNA，编码一个380个氨基酸的疏水蛋白（ShUT），与大鼠促进尿素转运蛋白UT-A2有66%的同源性。向异种卵母细胞注射ShUT cRNA诱导14c标记的尿素摄取增加10倍，被根皮素抑制（0.35 mM）。关闭mRNA在肾和脑中表达。相关mRNA种类存在于肝脏、血液、肾脏、鳃、肠、肌肉和直肠腺中。这是在海洋鱼类中首次发现的尿素转运体。我们认为，ShUT蛋白参与了角鲨肾小管的尿素重吸收，从而最大限度地减少了尿液中的尿素损失。
Marine elasmobranch fishes retain relatively high levels of urea to balance the osmotic stress of living in seawater. To maintain osmotic balance and reduce the energetic costs of making urea, it is important for these animals to minimize urea excretion to the environment. We have isolated a novel 2.2-kb cDNA from Squalus acanthias (spiny dogfish shark) kidney encoding a 380-amino acid hydrophobic protein (ShUT) with 66% identity to the rat facilitated urea transporter protein UT-A2. Injection of ShUT cRNA into Xenopusoocytes induced a 10-fold increase in14C-labeled urea uptake, inhibitable by phloretin (0.35 mM). ShUT mRNA is expressed in kidney and brain. Related mRNA species are found in liver, blood, kidney, gill, intestine, muscle, and rectal gland. This is the first facilitated urea transporter to be identified in a marine fish. We propose that the ShUT protein is involved in urea reabsorption by the renal tubules of the dogfish shark, which in turn minimizes urea loss in the urine.
影响因子：
15.9
作者：
SMITH, CP;LEE, WS;HEDIGER, MA
通讯作者：
HEDIGER, MA
大鼠内髓集合管最深亚段钠依赖性活性尿素分泌的证据。
DOI：
10.1172/jci1420
发表时间：
1998
期刊：
The Journal of clinical investigation
影响因子：
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作者：
Kato,A;Sands,JM
通讯作者：
Sands,JM