Chemical changes in rat leg muscle by phosphorus nuclear magnetic resonance.

Chemical changes in rat leg muscle by phosphorus nuclear magnetic resonance.
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磷核磁共振观察大鼠腿部肌肉的化学变化。

DOI:
10.1152/ajpcell.1985.248.5.c542
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发表时间:
1985
期刊:
The American journal of physiology
影响因子:
--
通讯作者:
Meyer,RA
Meyer,RA
中科院分区:
--
文献类型:
--
作者:
Kushmerick,MJ;Meyer,RA

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磷核磁共振光谱在145 MHz获得与表面线圈从腓肠肌跖肌麻醉大鼠。磷酸肌酸(PCr)和无机磷酸盐(Pi)的含量和细胞内的pH值进行了测定之前,期间和之后的收缩活动引起的抽搐或强直刺激的坐骨神经。PCr水平降低,Pi水平升高和细胞内酸化以分级方式实现,抽搐刺激速率从2 Hz增加到10 Hz,ATP含量无可检测变化。在所有情况下,PCr的减少与Pi含量的化学计量增加相匹配。PCr再合成的时间常数平均为30 s,比细胞内pH恢复到对照水平快5倍,并且与恢复开始时细胞内酸中毒的程度无关。
Phosphorus nuclear magnetic resonance spectra at 145 MHz were obtained with a surface coil from the gastrocnemius-plantaris muscles of anesthetized rats. Phosphocreatine (PCr) and inorganic phosphate (Pi) contents and intracellular pH were measured before, during, and after periods of contractile activity induced by twitch or tetanic stimulation of the sciatic nerve. Reduced levels of PCr, increased levels of Pi, and intracellular acidification were achieved in a graded fashion with increased rates of twitch stimulation from 2 to 10 Hz without detectable changes in the ATP content. In all cases, the decrease in PCr was matched by a stoichiometric increase in Pi content. The time constant of resynthesis of PCr averaged 30 s, was five times faster than the restoration of intracellular pH to control levels, and was independent of the degree of intracellular acidosis at the beginning of recovery.
与疲劳小鼠肌肉松弛减慢相关的代谢变化。
DOI: --
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