An adsorptive transfer technique coupled with brdicka reaction to reveal the importance of metallothionein in chemotherapy with platinum based cytostatics.
An adsorptive transfer technique coupled with brdicka reaction to reveal the importance of metallothionein in chemotherapy with platinum based cytostatics.
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DOI：
10.3390/ijms11124826
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发表时间：
2010
影响因子：
5.6
通讯作者：
Kizek R
中科院分区：
文献类型：
--
作者：
Krizkova S;Fabrik I;Huska D;Adam V;Babula P;Hrabeta J;Eckschlager T;Pochop P;Darsova D;Kukacka J;Prusa R;Trnkova L;Kizek R
基于铂金属的药物是最古老但也是最有效的化疗药物之一。许多临床研究表明，肿瘤细胞对药物的耐药性是化疗失败的常见原因。对于铂类药物，多药耐药性也可能与低分子量蛋白金属硫蛋白（MT）表达增加有关。本研究旨在研究MT与顺铂或卡铂的相互作用，采用吸附转移技术结合微分脉冲伏安法Brdicka反应（AdTS DPV Brdicka反应），并将体外结果与体内结果进行比较。体外研究结果显示铂类药物与 MT 之间具有很强的亲和力。此外，我们分析了用顺铂或卡铂处理的神经母细胞瘤细胞系的提取物。很明显，神经母细胞瘤 UKF-NB-4 顺铂耐药和顺铂敏感细胞系不太可能对铂基细胞抑制剂顺铂和卡铂的存在产生反应。最后，我们测定了接受卡铂治疗的兔子和接受相同药物治疗的视网膜母细胞瘤患者样本中的 MT 水平。
The drugs based on platinum metals represent one of the oldest, but also one of the most effective groups of chemotherapeutic agents. Thanks to many clinical studies it is known that resistance of tumor cells to drugs is a frequent cause of chemotherapy failure. With regard to platinum based drugs, multidrug resistance can also be connected with increased expression of low-molecular weight protein metallothionein (MT). This study aimed at investigating the interactions of MT with cisplatin or carboplatin, using the adsorptive transfer technique coupled with differential pulse voltammetry Brdicka reaction (AdTS DPV Brdicka reaction), and a comparison of in vitro results with results obtained in vivo. The results obtained from the in vitro study show a strong affinity between platinum based drugs and MT. Further, we analyzed extracts of neuroblastoma cell lines treated with cisplatin or carboplatin. It is clear that neuroblastoma UKF-NB-4 cisplatin-resistant and cisplatin-sensitive cell lines unlikely respond to the presence of the platinum-based cytostatics cisplatin and carboplatin. Finally, we determined the level of MT in samples from rabbits treated with carboplatin and patients with retinoblastoma treated with the same drug.
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影响因子：
50.5
作者：
Boyle, P;Ferlay, J
通讯作者：
Ferlay, J
影响因子：
6.1
作者：
Erk, M;Ivankovic, D;Pavicic, J
通讯作者：
Pavicic, J
影响因子：
5.1
作者：
Hou, Xin-fang;Fan, Qing-xia;Lu, Shi-xin
通讯作者：
Lu, Shi-xin
影响因子：
16.8
作者：
Damsma, Gerke E.;Alt, Aaron;Cramer, Patrick
通讯作者：
Cramer, Patrick
影响因子：
50.5
作者：
Bosetti, C.;Bertuccio, P.;La Vecchia, C.
通讯作者：
La Vecchia, C.