Innate immune imprints in SARS-CoV-2 Omicron variant infection convalescents.
Innate immune imprints in SARS-CoV-2 Omicron variant infection convalescents.
复制标题
SARS-CoV-2 Omicron 变异感染康复者的先天免疫印记
DOI：
10.1038/s41392-022-01237-y
复制
发表时间：
2022-11-16
影响因子：
39.3
通讯作者：
Cao, Xuetao
中科院分区：
文献类型：
--
作者：
Li, Zhiqing;Chen, Xiaosu;Dan, Junyan;Hu, Tianju;Hu, Ye;Liu, Shuxun;Chai, Yangyang;Shi, Yansong;Wu, Jian;Ni, Hailai;Zhu, Jiaqi;Wu, Yanfeng;Li, Nan;Yu, Yizhi;Wang, Zhongfang;Zhao, Jincun;Zhong, Nanshan;Ren, Xianwen;Shen, Zhongyang;Cao, Xuetao
在接种疫苗的人群中，SARS-CoV-2欧米克龙变异株感染通常会引起无症状到中度的新冠肺炎。免疫细胞可以通过接种疫苗和感染进行重新编程或“印记”，以产生针对后续挑战的保护性免疫。考虑到Omicron感染的免疫印记尚不清楚，我们通过单细胞RNA测序、表面蛋白质组图谱和血浆细胞因子定量来描绘人类Omicron感染的天然免疫图景。我们发现Omicron恢复期患者的免疫印记以单核细胞反应为主，IL-1β相关特征和干扰素反应特征分别表现为轻度和中度症状。低密度中性粒细胞增多，并表现出类似的IL-1、β相关和干扰素反应特征。轻度恢复期患者外周血中IL-1β、CCl_4、IL-9水平和PI3+中性粒细胞水平升高，提示偏向IL-1β应答；中度恢复期患者外周血中CXCL_(10)和干扰素应答单核细胞水平升高，提示干扰素应答持续。因此，髓系细胞的IL-1β或干扰素反应性可能预示奥米克龙感染的疾病严重程度，并介导冠状病毒感染后的病情。
SARS-CoV-2 Omicron variant infection generally gives rise to asymptomatic to moderate COVID-19 in vaccinated people. The immune cells can be reprogrammed or “imprinted” by vaccination and infections to generate protective immunity against subsequent challenges. Considering the immune imprint in Omicron infection is unclear, here we delineate the innate immune landscape of human Omicron infection via single-cell RNA sequencing, surface proteome profiling, and plasma cytokine quantification. We found that monocyte responses predominated in immune imprints of Omicron convalescents, with IL-1β-associated and interferon (IFN)-responsive signatures with mild and moderate symptoms, respectively. Low-density neutrophils increased and exhibited IL-1β-associated and IFN-responsive signatures similarly. Mild convalescents had increased blood IL-1β, CCL4, IL-9 levels and PI3+ neutrophils, indicating a bias to IL-1β responsiveness, while moderate convalescents had increased blood CXCL10 and IFN-responsive monocytes, suggesting durative IFN responses. Therefore, IL-1β- or IFN-responsiveness of myeloid cells may indicate the disease severity of Omicron infection and mediate post-COVID conditions.
登录
查看更多内容
影响因子：
7.3
作者：
Jang YJ;Lim JY;Kim S;Lee Y;Kweon MN;Kim JH
通讯作者：
Kim JH
DOI：
10.1056/nejmoa2202261
发表时间：
2022-06-02
期刊：
The New England journal of medicine
影响因子：
--
作者：
通讯作者：
--
影响因子：
8.8
作者：
Kimura I;Konno Y;Uriu K;Hopfensperger K;Sauter D;Nakagawa S;Sato K
通讯作者：
Sato K
影响因子：
15.9
作者：
Kessenbrock, Kai;Froehlich, Leopold;Jenne, Dieter E.
通讯作者：
Jenne, Dieter E.
影响因子：
64.8
作者：
La Manno G;Soldatov R;Zeisel A;Braun E;Hochgerner H;Petukhov V;Lidschreiber K;Kastriti ME;Lönnerberg P;Furlan A;Fan J;Borm LE;Liu Z;van Bruggen D;Guo J;He X;Barker R;Sundström E;Castelo-Branco G;Cramer P;Adameyko I;Linnarsson S;Kharchenko PV
通讯作者：
Kharchenko PV