Co‐localization of somatostatin mRNA and parvalbumin in the dorsal rat hippocampus after cerebral ischemia
Co‐localization of somatostatin mRNA and parvalbumin in the dorsal rat hippocampus after cerebral ischemia
复制标题
脑缺血后大鼠背侧海马生长抑素 mRNA 和小清蛋白的共定位
作者：
R. Bering;N. Diemer;A. Draguhn;F. Johansen
短暂性全脑缺血后，海马背区齿状筋膜中含有生长抑素的神经元大部分死亡，而CA1区的生长抑素能神经元存活。这些神经元对缺血的反应是短暂地减少生长抑素mRNA和肽的表达。我们已经验证了这种选择性脆弱性仅与那些不表达钙结合蛋白小白蛋白的生长抑素能神经元有关的假设。采用四血管闭塞模型研究大鼠全脑缺血10分钟后2天和16天海马背侧的缺血后变化。我们通过非放射性原位杂交和免疫细胞化学对生长抑素mRNA编码进行了双染色。齿状筋膜中只有5%的生长抑素能细胞含有细小蛋白。缺血后，生长抑素能细胞的数量永久性减少。在存活的神经元中，我们发现有或没有小蛋白表达的细胞。因此，小白蛋白的表达并不能预测牙筋膜中生长抑素能细胞的存活。相反，在CA1中，37%的生长抑素能细胞含有小蛋白。这些细胞不受短暂缺血期的影响。缺乏小白蛋白的生长抑素能细胞在第2天出现短暂的mRNA水平降低，但在第16天恢复到对照组水平。因此，钙缓冲蛋白parvalbumin的表达与CA1中生长抑素能神经元对缺血的短暂性影响的抵抗一致。
Following transient global ischemia most of the neurons containing somatostatin in the fascia dentata of the dorsal hippocampal formation die, while somatostatinergic neurons in the CA1 region survive. These neurons react to ischemia with a transiently reduced expression of somatostatin mRNA and peptide. We have tested the hypothesis that this selective vulnerability is solely related to those somatostatinergic neurons which do not express the calcium‐binding protein parvalbumin. Postischemic changes were studied in rat dorsal hippocampus at 2 and 16 days after 10 min of global cerebral ischemia using a four‐vessel occlusion model. We performed a double‐staining visualizing the mRNA coding for somatostatin by non‐radioactive in situ hybridization and parvalbumin protein by immunocytochemistry. Only 5% of the somatostatinergic cells in the fascia dentata contained parvalbumin. The number of somatostatinergic cells was permanently reduced following ischemia. Among surviving neurons we found cells with and without parvalbumin expression. Thus, expression of parvalbumin is not predictive for survival of somatostatinergic cells in the fascia dentata. In contrast, in CA1, 37% of the somatostatinergic cells contained parvalbumin. These cells were unaffected by the transient ischemic period. The somatostatinergic cells lacking parvalbumin showed transiently reduced mRNA levels at day 2, but recovered to control values at the 16th postischemic day. Thus, expression of the calcium‐buffering protein parvalbumin coincides with resistance of somatostatinergic neurons in CA1 to transient effects of ischemia.
影响因子：
56.9
作者：
SLOVITER, RS
通讯作者：
SLOVITER, RS
通过细胞内钙螯合保护齿状门细胞免受长期刺激。
DOI：
10.1126/science.2508225
发表时间：
1989
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Scharfman,HE;Schwartzkroin,PA
通讯作者：
Schwartzkroin,PA