The Role of Mitogen-Activated Protein (MAP) Kinase Signaling Components in the Fungal Development, Stress Response and Virulence of the Fungal Cereal Pathogen Bipolaris sorokiniana.
The Role of Mitogen-Activated Protein (MAP) Kinase Signaling Components in the Fungal Development, Stress Response and Virulence of the Fungal Cereal Pathogen Bipolaris sorokiniana.
复制标题
DOI：
10.1371/journal.pone.0128291
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Zhong S
中科院分区：
文献类型：
--
作者：
Leng Y;Zhong S
有丝分裂原活化蛋白激酶（MAPK）已被证明参与许多丝状植物病原真菌的真菌发育、有性繁殖、致病性和/或毒力，但谷类真菌病原体小离蠕孢（Bipolaris sorokiniana）中的MAPK基因尚未被表征。本研究在B的全基因组序列中鉴定了3个MAPK基因（CsSLT 2、CsHOG 1和CsFUS 3）和1个MAPK激酶激酶（MAPKKK）基因（CsST 11）的直向同源物。sorokiniana分离物ND90Pr，并为它们中的每一个产生敲除突变体。在大麦品种的非受伤叶片上，突变体BBCsfus3和BBCsste11在分生孢子和形成附着胞样结构方面有缺陷，表现出对氧化胁迫的敏感性，并且失去了致病性。鲍曼当接种在受伤的叶片鲍曼，pH7Csfus3和pH7Csste11突变体的毒性降低相比，野生型。与野生型相比，没有观察到形态学的变化，在Cshog 1突变体，但是，它们在氧化应激下的生长略有减少，对高渗胁迫高度敏感。Cshog1突变体形成正常的附着胞样结构，但在鲍曼叶片接种时，毒力降低。的cycsslt2突变体产生更多的营养菌丝，有较轻的色素沉着，细胞壁降解酶更敏感，并降低鲍曼叶片的毒力，虽然他们形成正常的附着胞像野生型。根感染试验表明，Δ Cshog 1和Δ Csslt 2突变体能够感染大麦根，而Δ Csfus 3和Δ Csset 11未能引起任何症状。然而，没有显着差异，观察到的毒力为p53 Cshog 1突变体，而p53 Csslt 2突变体表现出显着降低的毒力大麦根与野生型相比。我们的研究结果表明，所有这些MAPK和MAPKKK基因参与调节真菌的发展，在正常和胁迫条件下，并需要充分的毒性大麦植物。
Mitogen-activated protein kinases (MAPKs) have been demonstrated to be involved in fungal development, sexual reproduction, pathogenicity and/or virulence in many filamentous plant pathogenic fungi, but genes for MAPKs in the fungal cereal pathogen Bipolaris sorokiniana have not been characterized. In this study, orthologues of three MAPK genes (CsSLT2, CsHOG1 and CsFUS3) and one MAPK kinase kinase (MAPKKK) gene (CsSTE11) were identified in the whole genome sequence of the B. sorokiniana isolate ND90Pr, and knockout mutants were generated for each of them. The ∆Csfus3 and ∆Csste11 mutants were defective in conidiation and formation of appressoria-like structures, showed hypersensitivity to oxidative stress and lost pathogenicity on non-wounded leaves of barley cv. Bowman. When inoculated on wounded leaves of Bowman, the ∆Csfus3 and ∆Csste11 mutants were reduced in virulence compared to the wild type. No morphological changes were observed in the ∆Cshog1 mutants in comparison with the wild type; however, they were slightly reduced in growth under oxidative stress and were hypersensitive to hyperosmotic stress. The ∆Cshog1 mutants formed normal appressoria-like structures but were reduced in virulence when inoculated on Bowman leaves. The ∆Csslt2 mutants produced more vegetative hyphae, had lighter pigmentation, were more sensitive to cell wall degrading enzymes, and were reduced in virulence on Bowman leaves, although they formed normal appressoria like the wild type. Root infection assays indicated that the ∆Cshog1 and ∆Csslt2 mutants were able to infect barley roots while the ∆Csfus3 and ∆Csste11 failed to cause any symptoms. However, no significant difference in virulence was observed for ∆Cshog1 mutants while ∆Csslt2 mutants showed significantly reduced virulence on barley roots in comparison with the wild type. Our results indicated that all of these MAPK and MAPKKK genes are involved in the regulation of fungal development under normal and stress conditions and required for full virulence on barley plants.
登录
查看更多内容
DOI：
10.1016/j.bbapap.2005.07.032
发表时间：
2005-12-30
影响因子：
3.2
作者：
Heinisch, JJ
通讯作者：
Heinisch, JJ
影响因子：
3.3
作者：
Bahn, YS;Kojima, K;Heitman, J
通讯作者：
Heitman, J
影响因子：
3.5
作者：
Igbaria, Aeid;Lev, Sophie;Horwitz, Benjamin A.
通讯作者：
Horwitz, Benjamin A.
影响因子：
3.2
作者：
LILJEROTH, E;JANSSON, HB;SCHAFER, W
通讯作者：
SCHAFER, W
影响因子：
30.8
作者：
Friesen, Timothy L.;Stukenbrock, Eva H.;Oliver, Richard P.
通讯作者：
Oliver, Richard P.