FANCM Limits Meiotic Crossovers in Brassica Crops.
FANCM Limits Meiotic Crossovers in Brassica Crops.
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DOI：
10.3389/fpls.2018.00368
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发表时间：
2018
影响因子：
5.6
通讯作者：
Jenczewski E
中科院分区：
文献类型：
--
作者：
Blary A;Gonzalo A;Eber F;Bérard A;Bergès H;Bessoltane N;Charif D;Charpentier C;Cromer L;Fourment J;Genevriez C;Le Paslier MC;Lodé M;Lucas MO;Nesi N;Lloyd A;Chèvre AM;Jenczewski E
减数分裂交换（CO）是减数分裂过程中染色体正确分离和等位基因重排的必要条件。在WT植物中，CO的数量通常很小，这限制了植物育种程序可以捕获的遗传变异。这种限制的一部分是由FANCM等蛋白质造成的，FANCM的失活导致拟南芥中CO增加3倍。是否同样适用于作物需要确定。在这项研究中，我们确定了EMS诱导的突变FANCM在两个物种的经济相关的芸苔属。我们发现，在二倍体和四倍体油菜、芜菁和甘蓝型油菜中，fancm突变体的CO频率分别增加。在B。在芜菁中，我们观察到CO的数量增加了3倍，相当于以前在拟南芥中观察到的增加。在B。在欧洲油菜中，我们观察到在整倍体（AACC）和异源单倍体（AC）植物两者中较小但一致的增加（1.3倍）。在A. thaliana表明，在B中观察到的交叉频率增加较小。napus的FANCM突变体C拷贝的残余活性。总之，我们的研究结果表明，FANCM的抗CO活性在芸苔属中是保守的，开辟了新的途径，使更广泛的遗传多样性可用于作物改良。
Meiotic crossovers (COs) are essential for proper chromosome segregation and the reshuffling of alleles during meiosis. In WT plants, the number of COs is usually small, which limits the genetic variation that can be captured by plant breeding programs. Part of this limitation is imposed by proteins like FANCM, the inactivation of which results in a 3-fold increase in COs in Arabidopsis thaliana. Whether the same holds true in crops needed to be established. In this study, we identified EMS induced mutations in FANCM in two species of economic relevance within the genus Brassica. We showed that CO frequencies were increased in fancm mutants in both diploid and tetraploid Brassicas, Brassica rapa and Brassica napus respectively. In B. rapa, we observed a 3-fold increase in the number of COs, equal to the increase observed previously in Arabidopsis. In B. napus we observed a lesser but consistent increase (1.3-fold) in both euploid (AACC) and allohaploid (AC) plants. Complementation tests in A. thaliana suggest that the smaller increase in crossover frequency observed in B. napus reflects residual activity of the mutant C copy of FANCM. Altogether our results indicate that the anti-CO activity of FANCM is conserved across the Brassica, opening new avenues to make a wider range of genetic diversity accessible to crop improvement.
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影响因子：
12.3
作者：
Bauer E;Falque M;Walter H;Bauland C;Camisan C;Campo L;Meyer N;Ranc N;Rincent R;Schipprack W;Altmann T;Flament P;Melchinger AE;Menz M;Moreno-González J;Ouzunova M;Revilla P;Charcosset A;Martin OC;Schön CC
通讯作者：
Schön CC
影响因子：
7.4
作者：
Braatz, Janina;Harloff, Hans-Joachim;Jung, Christian
通讯作者：
Jung, Christian
影响因子：
4.5
作者：
Girard C;Chelysheva L;Choinard S;Froger N;Macaisne N;Lemhemdi A;Mazel J;Crismani W;Mercier R
通讯作者：
Mercier R
影响因子：
3.7
作者：
Gilchrist EJ;Sidebottom CH;Koh CS;Macinnes T;Sharpe AG;Haughn GW
通讯作者：
Haughn GW
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF