Intraspecific variation of recombination rate in maize.
Intraspecific variation of recombination rate in maize.
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DOI：
10.1186/gb-2013-14-9-r103
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发表时间：
2013
期刊：
影响因子：
12.3
通讯作者：
Schön CC
中科院分区：
文献类型：
--
作者：
Bauer E;Falque M;Walter H;Bauland C;Camisan C;Campo L;Meyer N;Ranc N;Rincent R;Schipprack W;Altmann T;Flament P;Melchinger AE;Menz M;Moreno-González J;Ouzunova M;Revilla P;Charcosset A;Martin OC;Schön CC
在有性繁殖生物体中，减数分裂交叉确保染色体的适当分离，并通过改组等位基因组合来促进遗传多样性。这种遗传重组在育种中被用来结合有利的等位基因，在遗传研究中被用来通过连锁或基于关联的方法来识别潜在的感兴趣的性状的遗传因素。杂交数量和沿染色体的分布在物种之间有所不同，但对它们的种内变异知之甚少。在这里，我们报告了属于Dent和Flint基因库的22个欧洲玉米自交系之间的重组率差异。我们用50k-SNP阵列对来自这些品系间杂交的23个双单倍体群体进行了基因分型，并构建了高密度遗传图谱，与玉米B73基因组序列拼接显示出良好的一致性。通过将每个遗传图谱与B73序列进行比对，我们获得了每个群体特有的染色体上的重组率。我们发现群体间基因组水平、染色体水平和染色体内水平的重组率存在显著差异，玉米品系间的基因组水平重组率也存在显著差异。使用双路径建模框架的交叉干扰分析揭示了重组率和干扰强度之间的负关联。据我们所知，本工作提供了关于真核生物中重组率和交叉干扰强度的种内变异的最全面的研究。重组率的差异将允许在杂交程序中选择高或低的重组系。我们的方法应该为精确识别玉米和其他生物中控制重组率的基因铺平道路。
In sexually reproducing organisms, meiotic crossovers ensure the proper segregation of chromosomes and contribute to genetic diversity by shuffling allelic combinations. Such genetic reassortment is exploited in breeding to combine favorable alleles, and in genetic research to identify genetic factors underlying traits of interest via linkage or association-based approaches. Crossover numbers and distributions along chromosomes vary between species, but little is known about their intraspecies variation. Here, we report on the variation of recombination rates between 22 European maize inbred lines that belong to the Dent and Flint gene pools. We genotype 23 doubled-haploid populations derived from crosses between these lines with a 50 k-SNP array and construct high-density genetic maps, showing good correspondence with the maize B73 genome sequence assembly. By aligning each genetic map to the B73 sequence, we obtain the recombination rates along chromosomes specific to each population. We identify significant differences in recombination rates at the genome-wide, chromosome, and intrachromosomal levels between populations, as well as significant variation for genome-wide recombination rates among maize lines. Crossover interference analysis using a two-pathway modeling framework reveals a negative association between recombination rate and interference strength. To our knowledge, the present work provides the most comprehensive study on intraspecific variation of recombination rates and crossover interference strength in eukaryotes. Differences found in recombination rates will allow for selection of high or low recombining lines in crossing programs. Our methodology should pave the way for precise identification of genes controlling recombination rates in maize and other organisms.
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影响因子：
5.4
作者：
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通讯作者：
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