Characterization and performance of a near-infrared 2-deoxyglucose optical imaging agent for mouse cancer models.
Characterization and performance of a near-infrared 2-deoxyglucose optical imaging agent for mouse cancer models.
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DOI：
10.1016/j.ab.2008.09.050
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发表时间：
2009-01-15
影响因子：
2.9
通讯作者：
Olive, D. Michael
中科院分区：
文献类型：
--
作者：
Kovar, Joy L.;Volcheck, William;Sevick-Muraca, Eva;Simpson, Melanie A.;Olive, D. Michael
关键词：
Malignant neoplasms exhibit an elevated rate of glycolysis over normal cells. This characteristic can be exploited for optical imaging of tumors in mice. A near infrared fluorophore, IRDye® 800CW, emission maximum 794 nm, was conjugated to 2-deoxyglucose (2-DG). An immunofluorescent cell-based assay was used to evaluate specificity and sensitivity of the conjugate in cultured cell monolayers. Dose dependent uptake was established with increasing concentrations of IRDye 800CW 2-DG for epithelial and prostate carcinomas. IRDye 800CW 2-DG was specifically blocked by an antibody against GLUT1 glucose transporter, and by excess unlabeled 2-DG or D-glucose. Signal was increased by a phorbol ester activator of glucose transport. Fluorescence microscopy data confirmed localization of the conjugate in the cytoplasm. Subsequent in vivo studies optimized dose, clearance, and timing for signal capture in nude mouse xenografts. In all cases, tumors were clearly imaged with good signal to noise characteristics. These data indicate that IRDye 800CW 2-DG is a broadly applicable optical imaging agent for in vivo imaging of neoplasms in mice.
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