STIM1 positively regulates the Ca2+ release activity of the inositol 1,4,5-trisphosphate receptor in bovine aortic endothelial cells.
STIM1 positively regulates the Ca2+ release activity of the inositol 1,4,5-trisphosphate receptor in bovine aortic endothelial cells.
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DOI：
10.1371/journal.pone.0114718
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Guillemette G
中科院分区：
文献类型：
--
作者：
Béliveau É;Lessard V;Guillemette G
血管内皮细胞积极参与心血管系统的许多功能，如调节动脉压、维持血流等。这些功能需要细胞内钙信号的多功能性，这是因为不同的信号可以通过改变钙响应的频率和幅度来编码。细胞利用细胞外和细胞内的钙离子池来调节细胞内的钙离子浓度。在非兴奋性细胞中，位于内质网(ER)上的三磷酸肌醇受体(IP3R)负责细胞内钙离子的释放。STIM1和STIM2蛋白也位于内质网，参与钙离子通道的激活。由于其钙敏感特性和与内质网上IP3R的亲和性，STIMS可以调节IP3R的活性。在本研究中，我们发现STIM1和STIM2在牛主动脉内皮细胞中表达，并且它们都与IP3R相互作用。STIM2对激动剂诱导的BAECs钙动员的调节作用较小，而STIM1通过促进SOCE和IP3R依赖的钙释放，在调节激动剂诱导的BAECs钙动员中发挥重要作用。
The endothelium is actively involved in many functions of the cardiovascular system, such as the modulation of arterial pressure and the maintenance of blood flow. These functions require a great versatility of the intracellular Ca2+ signaling that resides in the fact that different signals can be encoded by varying the frequency and the amplitude of the Ca2+ response. Cells use both extracellular and intracellular Ca2+ pools to modulate the intracellular Ca2+ concentration. In non-excitable cells, the inositol 1,4,5-trisphosphate receptor (IP3R), located on the endoplasmic reticulum (ER), is responsible for the release of Ca2+ from the intracellular store. The proteins STIM1 and STIM2 are also located on the ER and they are involved in the activation of a store-operated Ca2+ entry (SOCE). Due to their Ca2+ sensor property and their close proximity with IP3Rs on the ER, STIMs could modulate the activity of IP3R. In this study, we showed that STIM1 and STIM2 are expressed in bovine aortic endothelial cells and they both interact with IP3R. While STIM2 appears to play a minor role, STIM1 plays an important role in the regulation of agonist-induced Ca2+ mobilization in BAECs by a positive effect on both the SOCE and the IP3R-dependent Ca2+ release.
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DOI：
10.2741/3958
发表时间：
2012-01-01
期刊：
Frontiers in bioscience (Landmark edition)
影响因子：
--
作者：
Pani B;Bollimuntha S;Singh BB
通讯作者：
Singh BB
影响因子：
4.8
作者：
Li, Y;Wright, JM;Guggino, WB
通讯作者：
Guggino, WB
影响因子：
5.3
作者：
Smyth JT;Hwang SY;Tomita T;DeHaven WI;Mercer JC;Putney JW
通讯作者：
Putney JW
影响因子：
4.8
作者：
Antigny, Fabrice;Girardin, Nathalie;Frieden, Maud
通讯作者：
Frieden, Maud
影响因子：
4
作者：
Hirano, Katsuya;Hirano, Mayumi;Hanada, Akiko
通讯作者：
Hanada, Akiko