Field-evolved resistance to Bt maize by western corn rootworm.
Field-evolved resistance to Bt maize by western corn rootworm.
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DOI：
10.1371/journal.pone.0022629
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Dunbar MW
中科院分区：
文献类型：
--
作者：
Gassmann AJ;Petzold-Maxwell JL;Keweshan RS;Dunbar MW
每年在数百万公顷的土地上种植经过改造的作物，以产生来自苏云金芽孢杆菌（Bt）的杀虫毒素，从而减少了传统杀虫剂的使用并抑制了害虫。然而，抗性的进化可能会缩短这些好处。在美国，Bt玉米针对的主要害虫是西部玉米根虫（鞘翅目：玉米根萤叶甲科）。我们报告说，农民确定的领域有严重的根虫取食损伤Bt玉米包含西方玉米根虫的人口，显示出显着更高的生存率Cry 3Bb 1玉米在实验室生物测定比西方玉米根虫从外地没有相关的这种取食损伤。在所有情况下，经历严重根虫进食的田地含有Cry 3Bb 1玉米。对农民的采访表明，这些田地至少连续三年种植Cry 3Bb 1玉米。Cry 3Bb 1玉米在田间种植的年限与根虫种群在Cry 3Bb 1玉米上的存活率呈显著正相关。然而，在Cry 34/35 Ab 1玉米和Cry 3Bb 1玉米上存活的种群之间没有显著相关性，表明这些Bt毒素之间缺乏交叉抗性。这是第一次报告的外地演变的抗性Bt毒素的西方玉米根虫和任何种类的鞘翅目。保护地种植不足和抗性的非隐性遗传可能是导致抗性的原因。这些结果表明，改进抗性管理和更综合的方法来使用Bt作物可能是必要的。
Crops engineered to produce insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt) are planted on millions of hectares annually, reducing the use of conventional insecticides and suppressing pests. However, the evolution of resistance could cut short these benefits. A primary pest targeted by Bt maize in the United States is the western corn rootworm Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae). We report that fields identified by farmers as having severe rootworm feeding injury to Bt maize contained populations of western corn rootworm that displayed significantly higher survival on Cry3Bb1 maize in laboratory bioassays than did western corn rootworm from fields not associated with such feeding injury. In all cases, fields experiencing severe rootworm feeding contained Cry3Bb1 maize. Interviews with farmers indicated that Cry3Bb1 maize had been grown in those fields for at least three consecutive years. There was a significant positive correlation between the number of years Cry3Bb1 maize had been grown in a field and the survival of rootworm populations on Cry3Bb1 maize in bioassays. However, there was no significant correlation among populations for survival on Cry34/35Ab1 maize and Cry3Bb1 maize, suggesting a lack of cross resistance between these Bt toxins. This is the first report of field-evolved resistance to a Bt toxin by the western corn rootworm and by any species of Coleoptera. Insufficient planting of refuges and non-recessive inheritance of resistance may have contributed to resistance. These results suggest that improvements in resistance management and a more integrated approach to the use of Bt crops may be necessary.
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影响因子：
3.1
作者：
Coates, Brad S.;Sumerford, Douglas V.;Lewis, Leslie C.
通讯作者：
Lewis, Leslie C.
影响因子：
2
作者：
Jaffe, K;Issa, S;Haile, D
通讯作者：
Haile, D
DOI：
10.1073/pnas.0805565105
发表时间：
2008-12-09
影响因子：
11.1
作者：
Meihls, Lisa N.;Higdon, Matthew L.;Hibbard, Bruce E.
通讯作者：
Hibbard, Bruce E.
影响因子：
4.1
作者：
Carrière Y;Crowder DW;Tabashnik BE
通讯作者：
Tabashnik BE
影响因子：
4.1
作者：
Dhurua, Sanyasi;Gujar, Govind T.
通讯作者：
Gujar, Govind T.