The structural basis of transfer RNA mimicry and conformational plasticity by a viral RNA.
The structural basis of transfer RNA mimicry and conformational plasticity by a viral RNA.
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DOI：
10.1038/nature13378
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发表时间：
2014-07-17
期刊：
影响因子：
64.8
通讯作者：
Kieft, Jeffrey S.
中科院分区：
文献类型：
--
作者：
Colussi, Timothy M.;Costantino, David A.;Hammond, John A.;Ruehle, Grant M.;Nix, Jay C.;Kieft, Jeffrey S.
RNA可以说是功能最多样化的生物大分子。在某些情况下，单个离散的RNA序列执行多个角色，并且这可以由复杂的三维结构赋予。这种多功能性也可以通过给定RNA呈现不同构象（因此具有功能）状态的能力来驱动或增强。尽管其生物学的重要性，RNA结构驱动的多功能性的范例的详细结构的理解是缺乏的。解决这一缺口的例子是在单链正义RNA病毒中发现的，一个原型是在芜菁黄花叶病毒（TYMV）的3′端发现的tRNA样结构（TLS）。这种TLS不仅像tRNA一样驱动病毒基因组RNA（gRNA）的氨酰化，而且还与gRNA 3′非翻译区的其他结构相互作用，包含负链合成的启动子，并影响几个感染关键过程。这种TLS RNA可以让我们一窥RNA多功能性和可塑性的结构基础，但几十年来，它的高分辨率结构仍然难以捉摸。在这里，我们提出了完整的TYMV TLS的晶体结构，分辨率为2.0 μ m。在全球范围内，RNA采用模仿tRNA的形状，但它使用一组非常不同的分子内相互作用来实现这种形状。这些相互作用也允许TLS容易地转换构象。此外，TLS结构是“双面的”：一个“面”紧密模仿tRNA并驱动氨酰化，另一个“面”与tRNA不同并实现额外的功能。因此，TLS的结构执行几个功能，并与不同的结合伙伴，我们证明了它的能力，特异性结合到核糖体。
RNA is arguably the most functionally diverse biological macromolecule. In some cases a single discrete RNA sequence performs multiple roles and this can be conferred by a complex three-dimensional structure. This multifunctionality can also be driven or enhanced by the ability of a given RNA to assume different conformational (and therefore functional) states. Despite its biological importance, a detailed structural understanding of the paradigm of RNA structure-driven multifunctionality is lacking. Examples to address this gap are found in single-stranded positive-sense RNA viruses, a prototype being the tRNA-like structure (TLS) found at the 3′ end of the Turnip Yellow Mosaic Virus (TYMV). This TLS not only acts like a tRNA to drive aminoacylation of the viral genomic RNA (gRNA), but also interacts with other structures in the gRNA's 3′ untranslated region, contains the promoter for negative strand synthesis, and influences several infection-critical processes. This TLS RNA can provide a glimpse into the structural basis of RNA multifunctionality and plasticity, but for decades its high-resolution structure has remained elusive. Here, we present the crystal structure of the complete TYMV TLS to 2.0 Å resolution. Globally, the RNA adopts a shape that mimics tRNA, but it uses a very different set of intramolecular interactions to achieve this shape. These interactions also allow the TLS to readily switch conformations. In addition, the TLS structure is ‘two-faced’: one ‘face’ closely mimics tRNA and drives aminoacylation, the other ‘face’ diverges from tRNA and enables additional functionality. The TLS is thus structured to perform several functions and interact with diverse binding partners, and we demonstrate its ability to specifically bind to ribosomes.
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DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
5.4
作者：
Cliffe, Anna R.;Nash, Anthony A.;Dutia, Bernadette M.
通讯作者：
Dutia, Bernadette M.
影响因子：
2.9
作者：
DREHER, TW;TSAI, CH;GIEGE, R
通讯作者：
GIEGE, R
影响因子：
4.5
作者：
Costantino, D;Kieft, JS
通讯作者：
Kieft, JS
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH