Haploidy in somatic cells is induced by mature oocytes in mice.
Haploidy in somatic cells is induced by mature oocytes in mice.
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DOI：
10.1038/s42003-022-03040-5
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发表时间：
2022-01-25
影响因子：
5.9
通讯作者：
Kang E
中科院分区：
文献类型：
--
作者：
Lee Y;Trout A;Marti-Gutierrez N;Kang S;Xie P;Mikhalchenko A;Kim B;Choi J;So S;Han J;Xu J;Koski A;Ma H;Yoon JD;Van Dyken C;Darby H;Liang D;Li Y;Tippner-Hedges R;Xu F;Amato P;Palermo GD;Mitalipov S;Kang E
在配子中自然观察到单倍性;然而，在体细胞中实验诱导单倍性的尝试尚未成功。在这里，我们表明，成熟中期II（MII）逮捕的卵母细胞与细胞周期的G 0/G1阶段的体细胞核的减数分裂纺锤体的更换结果在从头纺锤体的形成，包括单染色单体的体细胞同源染色体组成。这种卵母细胞与精子受精触发一组同源染色体挤出到假极体（PPB）中，导致具有单倍体体细胞和精子原核（PN）的合子。培养后，18%的体细胞精子受精卵达到囊胚期，其中16%具有杂合二倍体基因组，包括体细胞单倍体和精子同源物在所有染色体上。我们还从体细胞精子胚胎中产生胚胎干细胞和活的后代。我们的发现可能为产生携带体细胞基因组的卵母细胞提供一种替代策略。Yeonmi Lee，Aysha Trout，Nuria Marti-Guiterrez等研究了小鼠去核卵母细胞中体细胞单倍体化的不同方面。他们的结果为产生携带体细胞基因组的卵母细胞提供了进一步的见解。
Haploidy is naturally observed in gametes; however, attempts of experimentally inducing haploidy in somatic cells have not been successful. Here, we demonstrate that the replacement of meiotic spindles in mature metaphases II (MII) arrested oocytes with nuclei of somatic cells in the G0/G1 stage of cell cycle results in the formation of de novo spindles consisting of somatic homologous chromosomes comprising of single chromatids. Fertilization of such oocytes with sperm triggers the extrusion of one set of homologous chromosomes into the pseudo-polar body (PPB), resulting in a zygote with haploid somatic and sperm pronuclei (PN). Upon culture, 18% of somatic-sperm zygotes reach the blastocyst stage, and 16% of them possess heterozygous diploid genomes consisting of somatic haploid and sperm homologs across all chromosomes. We also generate embryonic stem cells and live offspring from somatic-sperm embryos. Our finding may offer an alternative strategy for generating oocytes carrying somatic genomes. Yeonmi Lee, Aysha Trout, Nuria Marti-Guiterrez et al. examine different aspects of somatic cell haploidization in mouse enucleated oocytes. Their results provide further insight into generating oocytes carrying somatic genomes.
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影响因子：
14.9
作者：
The Gene Ontology Consortium
通讯作者：
The Gene Ontology Consortium
影响因子：
6.1
作者：
Mitalipov, S. M.;Zhou, Q.;Wolf, D. P.
通讯作者：
Wolf, D. P.
影响因子：
6.1
作者：
Fulka, J;Martinez, F;Tesarik, J
通讯作者：
Tesarik, J
影响因子：
64.8
作者：
Ma, Hong;Marti-Gutierrez, Nuria;Mitalipov, Shoukhrat
通讯作者：
Mitalipov, Shoukhrat
影响因子：
7
作者：
McKenna, Aaron;Hanna, Matthew;DePristo, Mark A.
通讯作者：
DePristo, Mark A.