Emodin blocks the SARS coronavirus spike protein and angiotensin-converting enzyme 2 interaction.
Emodin blocks the SARS coronavirus spike protein and angiotensin-converting enzyme 2 interaction.
复制标题
DOI：
10.1016/j.antiviral.2006.04.014
复制
发表时间：
2007-05
影响因子：
7.6
通讯作者：
Hsiang CY
中科院分区：
文献类型：
--
作者：
Ho TY;Wu SL;Chen JC;Li CC;Hsiang CY
严重急性呼吸系统综合征（SARS）是一种由新型冠状病毒（SARS- cov）引起的新型传染病。SARS-CoV刺突蛋白(S)是一种I型膜结合蛋白，对于病毒附着于宿主细胞受体血管紧张素转换酶2 （ACE2）至关重要。通过筛选台湾省中医药委员会监管的312种中草药，我们发现3种广泛使用的蓼科中草药抑制了SARS-CoV S蛋白与ACE2的相互作用。大黄根茎、何首乌根茎、何首乌藤的IC50值在1 ~ 10 μg/ml之间。大黄素是一种从大黄属和蓼属中提取的蒽醌类化合物，其抑制S蛋白和ACE2的相互作用呈剂量依赖性。它还能抑制S蛋白伪型逆转录病毒对Vero E6细胞的感染性。这些发现提示大黄素可能被认为是治疗SARS的潜在先导药物。
Severe acute respiratory syndrome (SARS) is an emerging infectious disease caused by a novel coronavirus (SARS-CoV). SARS-CoV spike (S) protein, a type I membrane-bound protein, is essential for the viral attachment to the host cell receptor angiotensin-converting enzyme 2 (ACE2). By screening 312 controlled Chinese medicinal herbs supervised by Committee on Chinese Medicine and Pharmacy at Taiwan, we identified that three widely used Chinese medicinal herbs of the family Polygonaceae inhibited the interaction of SARS-CoV S protein and ACE2. The IC50 values for Radix et Rhizoma Rhei (the root tubers of Rheum officinale Baill.), Radix Polygoni multiflori (the root tubers of Polygonum multiflorum Thunb.), and Caulis Polygoni multiflori (the vines of P. multiflorum Thunb.) ranged from 1 to 10 μg/ml. Emodin, an anthraquinone compound derived from genus Rheum and Polygonum, significantly blocked the S protein and ACE2 interaction in a dose-dependent manner. It also inhibited the infectivity of S protein-pseudotyped retrovirus to Vero E6 cells. These findings suggested that emodin may be considered as a potential lead therapeutic agent in the treatment of SARS.
登录
查看更多内容
影响因子：
64.8
作者：
Li W;Moore MJ;Vasilieva N;Sui J;Wong SK;Berne MA;Somasundaran M;Sullivan JL;Luzuriaga K;Greenough TC;Choe H;Farzan M
通讯作者：
Farzan M
DOI：
10.1016/j.bbrc.2003.11.180
发表时间：
2004-01-23
影响因子：
3.1
作者：
Ho TY;Wu SL;Cheng SE;Wei YC;Huang SP;Hsiang CY
通讯作者：
Hsiang CY
影响因子：
7.6
作者：
Cinatl J Jr;Michaelis M;Hoever G;Preiser W;Doerr HW
通讯作者：
Doerr HW
影响因子：
6.4
作者：
Greenough, TC;Babcock, GJ;Ambrosino, DM
通讯作者：
Ambrosino, DM
影响因子：
5.4
作者：
Bosch, BJ;van der Zee, R;Rottier, PJM
通讯作者：
Rottier, PJM