Structure of vagal afferent nerve terminal fibers in the mouse trachea.
Structure of vagal afferent nerve terminal fibers in the mouse trachea.
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DOI：
10.1016/j.resp.2018.01.001
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发表时间：
2018-03
影响因子：
2.3
通讯作者：
Kollarik M
中科院分区：
文献类型：
--
作者：
Hennel M;Harsanyiova J;Ru F;Zatko T;Brozmanova M;Trancikova A;Tatar M;Kollarik M
初级传入神经末梢的结构深刻地影响着它们的功能。虽然复杂的迷走神经终末(牵张感受器、咳嗽感受器和神经上皮体)已经被完全描述，但对于不形成不同复杂终末(通常被称为游离神经纤维)的呼吸道神经的结构却知之甚少。我们通过AAV-GFP病毒载体的转染法选择性地诱导GFP在小鼠迷走传入神经中的表达，并通过整体器官共聚焦成像显示气管内的神经末梢。根据结构特征，我们在气管内识别出四种类型的迷走神经传入神经纤维终末。重要的是，我们发现不同的气管组织间隔是由不同类型的神经纤维以不重叠的方式支配的。因此，独立的终末类型支配气管上皮、含有软骨环和韧带的气管前外侧壁以及含有平滑肌的背壁。我们的结果将有助于迷走神经传入神经结构-功能关系的研究和呼吸反射的调节。
The structure of primary afferent nerve terminals profoundly influences their function. While the complex vagal airway nerve terminals (stretch receptors, cough receptors and neuroepithelial bodies) were thoroughly characterized, much less is known about the structure of airway nerves that do not form distinct complex terminals (often termed free nerve fibers). We selectively induced expression of GFP in vagal afferent nerves in the mouse by transfection with AAV-GFP virus vector and visualized nerve terminals in the trachea by whole organ confocal imaging. Based on structural characteristics we identified four types of vagal afferent nerve fiber terminals in the trachea. Importantly, we found that distinct compartments of tracheal tissue are innervated by distinct nerve fiber terminal types in a non-overlapping manner. Thus, separate terminal types innervate tracheal epithelium vs. anterolateral tracheal wall containing cartilaginous rings and ligaments vs. dorsal wall containing smooth muscle. Our results will aid the study of structure-function relationships in vagal airway afferent nerves and regulation of respiratory reflexes.
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影响因子：
5.5
作者：
Kollarik, M.;Carr, M. J.;Undem, B. J.
通讯作者：
Undem, B. J.
影响因子：
3.3
作者：
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通讯作者：
Canning, Brendan J.
影响因子：
5.6
作者：
Nassenstein, Christina;Wiegand, Silke;Kummer, Wolfgang
通讯作者：
Kummer, Wolfgang
影响因子：
3.3
作者：
Watanabe, N.;Horie, S.;Priestley, J. V.
通讯作者：
Priestley, J. V.
影响因子：
2.5
作者：
BALUK, P;NADEL, JA;MCDONALD, DM
通讯作者：
MCDONALD, DM