Structural insights into the Venus flytrap mechanosensitive ion channel Flycatcher1.
Structural insights into the Venus flytrap mechanosensitive ion channel Flycatcher1.
复制标题
DOI：
10.1038/s41467-022-28511-5
复制
发表时间：
2022-02-14
影响因子：
16.6
通讯作者：
Ward AB
中科院分区：
文献类型：
--
作者：
Jojoa-Cruz S;Saotome K;Tsui CCA;Lee WH;Sansom MSP;Murthy SE;Patapoutian A;Ward AB
Flycatcher1 (FLYC1) 是一种 MScS 同源物，最近被确定为参与捕蝇草猎物识别的候选机械敏感 (MS) 离子通道。 FLYC1 是一种较大的蛋白质，其序列与之前研究的 MScS 同源物不同，这表明它具有有助于其功能的独特结构特征。在这里，我们通过冷冻电子显微镜、分子动力学模拟和电生理学来表征 FLYC1。与细菌 MscS 和植物 MSL1 通道类似，我们发现 FLYC1 中央核心包括细胞质笼中的侧门，通过识别调节通道电导的关键残基来调节离子偏好和传导。拓扑独特的细胞质侧翼区域可以采用“向上”或“向下”构象，使通道不对称。向上构象特异性相互作用的破坏会严重延迟通道失活 40 倍，这可能是由于通道开放状态的稳定所致。我们的结果说明了调节 FLYC1 机械门控的新颖结构特征和可能的构象转变。 Flycatcher1 (FLYC1) 是一种候选机械敏感通道，参与维纳斯捕蝇草触摸诱导的猎物捕获。在这里，作者报告了 FLYC1 的结构和功能细节，以及对门控构象转变的见解。
Flycatcher1 (FLYC1), a MscS homolog, has recently been identified as a candidate mechanosensitive (MS) ion channel involved in Venus flytrap prey recognition. FLYC1 is a larger protein and its sequence diverges from previously studied MscS homologs, suggesting it has unique structural features that contribute to its function. Here, we characterize FLYC1 by cryo-electron microscopy, molecular dynamics simulations, and electrophysiology. Akin to bacterial MscS and plant MSL1 channels, we find that FLYC1 central core includes side portals in the cytoplasmic cage that regulate ion preference and conduction, by identifying critical residues that modulate channel conductance. Topologically unique cytoplasmic flanking regions can adopt ‘up’ or ‘down’ conformations, making the channel asymmetric. Disruption of an up conformation-specific interaction severely delays channel deactivation by 40-fold likely due to stabilization of the channel open state. Our results illustrate novel structural features and likely conformational transitions that regulate mechano-gating of FLYC1. Flycatcher1 (FLYC1) is a candidate mechanosensitive channel involved in Venus flytrap touch-induced prey capture. Here, the authors report structural and functional details of FLYC1, with insights into gating conformational transitions.
登录
查看更多内容
影响因子：
48
作者：
Barad BA;Echols N;Wang RY;Cheng Y;DiMaio F;Adams PD;Fraser JS
通讯作者：
Fraser JS
DOI：
10.1107/s2059798318009324
发表时间：
2018-09-01
期刊：
Acta crystallographica. Section D, Structural biology
影响因子：
--
作者：
Afonine PV;Klaholz BP;Moriarty NW;Poon BK;Sobolev OV;Terwilliger TC;Adams PD;Urzhumtsev A
通讯作者：
Urzhumtsev A
DOI：
10.4161/chan.20998
发表时间：
2012-07
期刊：
Channels (Austin, Tex.)
影响因子：
--
作者：
Edwards MD;Black S;Rasmussen T;Rasmussen A;Stokes NR;Stephen TL;Miller S;Booth IR
通讯作者：
Booth IR
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
16.6
作者：
Cox, C. D.;Nomura, T.;Martinac, B.
通讯作者：
Martinac, B.