Glycogene expression profiles from a HaCaT cell line stably transfected with HPV16 E5 oncogene.
Glycogene expression profiles from a HaCaT cell line stably transfected with HPV16 E5 oncogene.
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稳定表达HPV16E5癌基因的HaCaT细胞株的糖基因表达谱
DOI：
10.3892/mmr.2020.11630
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发表时间：
2020-12
影响因子：
3.4
通讯作者：
Vallejo-Ruiz V
中科院分区：
文献类型：
--
作者：
Cisneros-Ramírez D;Martínez-Laguna Y;Martínez-Morales P;Aguilar-Lemarroy A;Jave-Suárez LF;Santos-López G;Reyes-Leyva J;Vallejo-Ruiz V
据报道，在宫颈转化过程中聚糖抗原的表达发生改变，表明某些糖基因的mRNA水平增加。人乳头瘤病毒（HPV）是宫颈癌的病原体。高危型HPV E5被认为是一种致癌基因，并与细胞转化有关。E6和E7 HPV癌蛋白修饰某些糖基因的表达。E5 HPV蛋白在糖原表达变化中的作用尚未报道。本研究的目的是确定HPV 16 E5癌蛋白对糖基因表达的影响。对于这些，使用HaCaT细胞系进行微阵列测定，并鉴定改变的糖原。通过逆转录定量PCR（RT-qPCR）测定某些糖基因的mRNA水平。使用计算机模拟分析，本研究确定糖基化途径被E5改变。微阵列分析揭示了某些糖基因的改变，包括ST 6 GAL 1、ST 3GAL 3、CHST 2和MANBA的上调，以及UGT 2B 15、GALNT 11、NDST 2和UGT 1A 10的下调。通过RT-qPCR证实唾液酸转移酶基因的mRNA水平增加。此外，进行计算机模拟分析，以鉴定在E5癌蛋白存在下改变的糖基化网络。分析表明，E5可以修饰聚糖唾液酸化、N-糖基化途径、硫酸角质素和糖胺聚糖合成。据我们所知，目前的研究是第一个确定HPV 16 E5癌蛋白在糖基因表达变化中的作用。结果表明，在癌前和恶性宫颈组织中报告的唾液酸转移酶mRNA水平增加可能是E5癌蛋白表达的结果。结果提供了一个可能的作用，HPV感染的糖基化的变化报告在宫颈转化。
The altered expression of glycan antigens has been reported during cervix transformation, demonstrating increased mRNA levels of certain glycogenes. Human papillomavirus (HPV) is the aetiological agent of cervical cancer. High risk HPV E5 is considered an oncogene and has been implicated in cell transformation. E6 and E7 HPV oncoproteins modify the expression of certain glycogenes. The role of the E5 HPV protein in glycogene expression changes has not yet been reported. The aim of the present study was to determine the effects of HPV16 E5 oncoprotein on glycogene expression. For these, a microarray assay was performed using the HaCaT cell line and altered glycogenes were identified. The mRNA levels of certain glycogenes were determined via reverse transcription-quantitative PCR (RT-qPCR). Using in silico analysis, the present study identified that glycosylation pathways were altered by E5. Microarray analysis revealed alterations in certain glycogenes, including the upregulation of ST6GAL1, ST3GAL3, CHST2 and MANBA, and the downregulation of UGT2B15, GALNT11, NDST2 and UGT1A10. Increased mRNA levels were confirmed via RT-qPCR for sialyltransferases genes. Additionally, in silico analysis was performed to identify glycosylation networks altered in the presence of the E5 oncoprotein. The analysis revealed that E5 could modify glycan sialylation, the N-glycosylation pathway, keratan sulfate and glycosaminoglycan synthesis. To the best of our knowledge, the current study was the first to determine the role of the HPV16 E5 oncoprotein in glycogene expression changes. The results indicated that increased sialyltransferase mRNA levels reported in pre-malignant and malignant cervical tissues could be the result of E5 oncoprotein expression. The results provide a possible role of HPV infection on glycosylation changes reported during cervix transformation.
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影响因子：
3.7
作者：
Aco-Tlachi M;Carreño-López R;Martínez-Morales PL;Maycotte P;Aguilar-Lemarroy A;Jave-Suárez LF;Santos-López G;Reyes-Leyva J;Vallejo-Ruiz V
通讯作者：
Vallejo-Ruiz V
影响因子：
4.8
作者：
Deligny, Audrey;Dierker, Tabea;Kjellen, Lena
通讯作者：
Kjellen, Lena
影响因子：
4.6
作者：
Ashkani J;Naidoo KJ
通讯作者：
Naidoo KJ
影响因子：
5.4
作者：
CONRAD, M;BUBB, VJ;SCHLEGEL, R
通讯作者：
SCHLEGEL, R
影响因子：
5.4
作者：
Kabsch, K;Alonso, A
通讯作者：
Alonso, A