Glycogene expression profiles based on microarray data from cervical carcinoma HeLa cells with partially silenced E6 and E7 HPV oncogenes.
Glycogene expression profiles based on microarray data from cervical carcinoma HeLa cells with partially silenced E6 and E7 HPV oncogenes.
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DOI：
10.1186/s13027-018-0197-2
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发表时间：
2018
影响因子：
3.7
通讯作者：
Vallejo-Ruiz V
中科院分区：
文献类型：
--
作者：
Aco-Tlachi M;Carreño-López R;Martínez-Morales PL;Maycotte P;Aguilar-Lemarroy A;Jave-Suárez LF;Santos-López G;Reyes-Leyva J;Vallejo-Ruiz V
异常糖基化是肿瘤细胞的特征。某些糖链结构的表达与预后不良有关。在宫颈癌中，某些糖基因表达水平的变化与淋巴侵袭有关。人乳头瘤病毒(HPV)感染是宫颈癌发生发展的重要因素之一。HPV癌蛋白E6和E7与宫颈癌的发生有关，可以改变宿主基因的表达谱。这些癌蛋白在糖基化改变中的作用以前还没有报道。为了确定E6和E7癌蛋白对糖基因表达的影响，我们在HeLa细胞中部分沉默了E6和E7癌基因，我们进行了微阵列表达分析以确定改变的糖基因，并通过RT-qPCR定量检测了糖基因的mRNA水平。构建了一个蛋白质-蛋白质相互作用网络，以确定可能改变的糖基化途径。基因芯片分析显示，HeLa shE6/E7细胞中有9个糖基因表达上调，7个糖基因表达下调。其中一些基因参与与Notch蛋白和O-糖链抗原相关的糖基化。我们的结果支持E6和E7癌蛋白可以改变糖基因的表达，其产物参与与增殖、黏附和凋亡有关的结构的合成。
Aberrant glycosylation is a characteristic of tumour cells. The expression of certain glycan structures has been associated with poor prognosis. In cervical carcinoma, changes in the expression levels of some glycogenes have been associated with lymph invasion. Human papillomavirus (HPV) infection is one of the most important factors underlying the development of cervical cancer. The HPV oncoproteins E6 and E7 have been implicated in cervical carcinogenesis and can modify the host gene expression profile. The roles of these oncoproteins in glycosylation changes have not been previously reported. To determine the effect of the E6 and E7 oncoproteins on glycogene expression we partially silenced the E6 and E7 oncogenes in HeLa cells, we performed a microarray expression assay to identify altered glycogenes and quantified the mRNA levels of glycogenes by RT-qPCR. A protein-protein interaction network was constructed to identify potentially altered glycosylation pathways. The microarray analysis showed 9 glycogenes that were upregulated and 7 glycogenes that were downregulated in HeLa shE6/E7 cells. Some of these genes participate in glycosylation related to Notch proteins and O-glycans antigens. Our results support that E6 and E7 oncoproteins could modify glycogene expression the products of which participate in the synthesis of structures implicated in proliferation, adhesion and apoptosis.
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影响因子：
--
作者：
Huang MJ;Hu RH;Chou CH;Hsu CL;Liu YW;Huang J;Hung JS;Lai IR;Juan HF;Yu SL;Wu YM;Huang MC
通讯作者：
Huang MC
DOI：
10.1007/978-1-4614-0899-4_15
发表时间：
2012-01-01
期刊：
NOTCH SIGNALING IN EMBRYOLOGY AND CANCER
影响因子：
--
作者：
Dang, Thao P.
通讯作者：
Dang, Thao P.
影响因子：
10.5
作者：
Gewin, L;Myers, H;Galloway, DA
通讯作者：
Galloway, DA
MAN2A1-FER融合基因由人肝脏和其他肿瘤类型表达，并且在小鼠中具有致癌活性。
影响因子：
29.4
作者：
Chen ZH;Yu YP;Tao J;Liu S;Tseng G;Nalesnik M;Hamilton R;Bhargava R;Nelson JB;Pennathur A;Monga SP;Luketich JD;Michalopoulos GK;Luo JH
通讯作者：
Luo JH
DOI：
10.4081/ejh.2014.2334
发表时间：
2014-05-26
期刊：
European journal of histochemistry : EJH
影响因子：
--
作者：
Clark AT;Guimarães da Costa VM;Bandeira Costa L;Bezerra Cavalcanti CL;De Melo Rêgo MJ;Beltrão EI
通讯作者：
Beltrão EI