Pattern-recognition receptors in duck (Anas platyrhynchos): identification, expression and function analysis of toll-like receptor 3
Pattern-recognition receptors in duck (Anas platyrhynchos): identification, expression and function analysis of toll-like receptor 3
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鸭（Anas platyrhynchos）模式识别受体：Toll样受体3的鉴定、表达和功能分析
DOI：
10.1080/00071668.2020.1853045
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发表时间：
2020-11
影响因子：
2
通讯作者：
Lu L.
中科院分区：
文献类型：
--
作者：
Gu T.;Li G.;Wu X.;Zeng T.;Xu Q.;Li L.;Vladyslav S.;Chen G.;Lu L.
抽象的1。先天免疫通过被称为模式识别受体（PRRs）的无数种系编码受体，为抵御病原生物提供了第一道防线。toll样受体（Toll-like receptor， TLR） 3是PRRs的重要成员，通过识别病毒源性rna，在宿主防御病毒感染中发挥着不可或缺的作用。然而，对于禽流感病毒的天然宿主鸭（Anas platyrhynchos）中TLR3的结构和功能知之甚少。2. 本研究利用反转录聚合酶链反应（RT-PCR）快速扩增cDNA末端（RACE）克隆了鸭TLR3的全长cDNA。鸭TLR3 cDNA序列全长4046 bp，编码895个氨基酸。多重序列比对表明，鸭的TLR3与其他脊椎动物的TLR3具有较高的相似性。3. 实时荧光定量PCR （Quantitative real-time PCR, qRT-PCR）分析显示，TLR3 mRNA在所有被测组织中均组成性表达，在肾脏、肝脏、胸肌、卵巢和心脏中表达水平较高。在病毒或细菌模拟物（包括LPS、poly（I:C）、pam3CSK4、FLS-1、FLA-ST和R848）刺激后，TLR3转录物显著上调。同时，鸭TLR3的过表达显著促进poly（I:C）刺激后IFN-β、IRF7、TRIF、Mx、STAT1和STAT2 mRNA的转录。4. 这些结果表明，TLR3在鸭对病毒和细菌感染的抗性中起重要作用。
ABSTRACT 1. Innate immunity provides the first line of defence against pathogenic organisms through a myriad of germline encoded receptors called pattern-recognition receptors (PRRs). Toll-like receptor (TLR) 3, as an important member of PRRs, is indispensable for host defence against viral infection by recognising virus-derived RNAs. However, little is known about the structure and function of TLR3 in ducks (Anas platyrhynchos), a natural host for the avian influenza virus. 2. This study cloned the full-length cDNA of duck TLR3 using reverse transcription polymerase chain reaction (RT-PCR) with rapid amplification of cDNA ends (RACE). The cDNA sequence of duck TLR3 was 4046 bp in length and encoded 895 amino acids. Multiple sequence alignment showed that duck TLR3 shared high similarity with that from other vertebrates. 3. Quantitative real-time PCR (qRT-PCR) analysis suggested that TLR3 mRNA was constitutively expressed in all tissues tested, having higher levels in the kidney, liver, breast muscle, ovary and heart. After stimulation with viral- or bacterial-mimics, including LPS, poly(I:C), pam3CSK4, FLS-1, FLA-ST and R848, the TLR3 transcript was significantly upregulated. Meanwhile, overexpression of duck TLR3 significantly promoted the transcription of IFN-β, IRF7, TRIF, Mx, STAT1 and STAT2 mRNA after stimulation with poly(I:C). 4. These results suggested that TLR3 play an important role in resistance against viral and bacterial infections in ducks.
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DOI：
10.4049/jimmunol.0903944
发表时间：
2010-06-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Sweeney SE;Kimbler TB;Firestein GS
通讯作者：
Firestein GS
影响因子：
64.8
作者：
Alexopoulou, L;Holt, AC;Flavell, RA
通讯作者：
Flavell, RA
影响因子：
4.4
作者：
T. Sugiyama;K. Hoshino;M. Saito;T. Yano;I. Sasaki;C. Yamazaki;S. Akira;T. Kaisho
通讯作者：
T. Sugiyama;K. Hoshino;M. Saito;T. Yano;I. Sasaki;C. Yamazaki;S. Akira;T. Kaisho
影响因子：
1.8
作者：
Iqbal, M;Philbin, VJ;Smith, AL
通讯作者：
Smith, AL
影响因子：
29.7
作者：
K. Takeda;T. Kaisho;S. Akira
通讯作者：
K. Takeda;T. Kaisho;S. Akira