Characterization of the propanil biodegradation pathway in Sphingomonas sp. Y57 and cloning of the propanil hydrolase gene prpH.
Characterization of the propanil biodegradation pathway in Sphingomonas sp. Y57 and cloning of the propanil hydrolase gene prpH.
复制标题
鞘氨醇单胞菌中敌稗生物降解途径的表征。
DOI：
10.1016/j.jhazmat.2011.09.040
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发表时间：
2011-11
影响因子：
13.6
通讯作者：
Shun-Peng Li
中科院分区：
文献类型：
--
作者：
Ji Zhang;Ji-Quan Sun;Qiao-Yun Yuan;Chao Li;Xin Yan*;Qing Hong*;Shun-Peng Li
本课题组前期从某除草剂厂废水处理系统中分离到一株异丙隆降解菌Sphingomonasp.Y57。有趣的是，该菌株还显示出降解敌稗（3，4-二氯丙酰胺）的能力。目前的工作表明，Y57通过以下途径降解敌稗：敌稗首先水解为3，4-二氯苯胺（3，4-DCA），然后转化为4，5-二氯邻苯二酚，然后进行芳环断裂和进一步加工。3，4-DCA也发生了N-酰化和N-脱酰化反应，并在N-酰化产物中首次发现了3，4-二氯丙酰苯胺。从Y57中克隆了编码敌稗水解酶的基因，该基因负责将敌稗转化为3，4-DCA，并命名为prpH。在大肠杆菌BL21中表达PrpH，用Ni-次氮基三乙酸亲和层析纯化。PrpH在40 ° C和pH 7.0下显示出对敌稗的最高活性。还测定了金属离子对PrpH的敌稗降解活性的影响。据我们所知，这是第一个报告的菌株，可以降解敌稗和3，4-DCA和第一个鉴定的基因编码的敌稗水解酶的细菌。
In our previous study, the isoproturon-degrading strain Sphingomonas sp. Y57 was isolated from the wastewater treatment system of an herbicide factory. Interestingly, this strain also showed the ability to degrade propanil (3,4-dichloropropionamilide). The present work reveals that Y57 degrades propanil via the following pathway: propanil was initially hydrolyzed to 3,4-dichloroaniline (3,4-DCA) and then converted to 4,5-dichlorocatechol, which was then subjected to aromatic ring cleavage and further processing. N-acylation and N-deacylation of 3,4-DCA also occurred, and among N-acylation products, 3,4-dichloropropionanilide was found for the first time. The gene encoding the propanil hydrolase responsible for transforming propanil into 3,4-DCA was cloned from Y57 and was designated as prpH. PrpH was expressed in Escherichia coli BL21 and purified using Ni-nitrilotriacetic acid affinity chromatography. PrpH displayed the highest activity against propanil at 40°C and at pH 7.0. The effect of metal ions on the propanil-degrading activity of PrpH was also determined. To our knowledge, this is the first report of a strain that can degrade both propanil and 3,4-DCA and the first identification of a gene encoding a propanil hydrolase in bacteria.
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DOI：
10.1186/1472-6904-9-3
发表时间：
2009-02-16
期刊：
BMC clinical pharmacology
影响因子：
--
作者：
Roberts DM;Heilmair R;Buckley NA;Dawson AH;Fahim M;Eddleston M;Eyer P
通讯作者：
Eyer P
影响因子：
4.1
作者：
P. Wilson;J. F. Foos
通讯作者：
P. Wilson;J. F. Foos
影响因子：
13.6
作者：
Ji-Quan Sun;Lian Xu;Yue-qin Tang;Fu‐ming Chen;Wei-Qiang Liu;Xiao‐Lei Wu
通讯作者：
Ji-Quan Sun;Lian Xu;Yue-qin Tang;Fu‐ming Chen;Wei-Qiang Liu;Xiao‐Lei Wu
影响因子：
12.8
作者：
Cerejeira, MJ;Viana, P;Silva-Fernandes, AM
通讯作者：
Silva-Fernandes, AM
影响因子：
3.2
作者：
Hildmann, C;Ninkovic, M;Schwienhorst, A
通讯作者：
Schwienhorst, A