Isolation and expression of Pax6 and atonal homologues in the American horseshoe crab, Limulus polyphemus.

Isolation and expression of Pax6 and atonal homologues in the American horseshoe crab, Limulus polyphemus.
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美国马蹄蟹Limulus Polyphemus中Pax6和Atonal同源物的分离和表达。

DOI:
10.1002/dvdy.21634
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发表时间:
2008-08
影响因子:
2.5
通讯作者:
Brown, Nadean L.
Brown, Nadean L.
中科院分区:
生物学3区
文献类型:
--
作者:
Blackburn, David C.;Conley, Kevin W.;Plachetzki, David C.;Kempler, Karen;Battelle, Barbara-Anne;Brown, Nadean L.

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Pax6调节许多动物的眼睛发育。此外,Pax6激活无脊椎动物和脊椎动物眼睛中的无调转录因子。在这里,我们调查的作用Pax6和无调在胚胎发育的美洲鲎不发达的外侧,内侧和腹侧的眼睛,以及启动外侧小眼和内侧单眼的形成。鲎的眼睛发育特别令人感兴趣,因为这些动物在节肢动物系统发育中占有独特的地位,并且拥有多种眼睛类型。此外,眼睛发育的分子基础还有待于在螯合动物中进行研究。我们的特点是鲎Pax6基因,多个剪接产物和预测的蛋白质亚型,和一个无调同源。出乎意料的是,这两种基因都没有在发育中的眼睛类型中表达,尽管这两种基因都存在于侧感觉器官中,这是一种功能未知的结构。
Pax6 regulates eye development in many animals. In addition, Pax6 activates atonal transcription factors in both invertebrate and vertebrate eyes. Here we investigate the roles of Pax6 and atonal during embryonic development of Limulus polyphemus rudimentary lateral, medial and ventral eyes, and the initiation of lateral ommatidial eye and medial ocelli formation. Limulus eye development is of particular interest because these animals hold a unique position in arthropod phylogeny and possess multiple eye types. Furthermore, the molecular underpinnings of eye development have yet to be investigated in chelicerates. We characterized a Limulus Pax6 gene, with multiple splice products and predicted protein isoforms, and one atonal homologue. Unexpectedly, neither gene is expressed in the developing eye types examined, although both genes are present in the lateral sense organ, a structure of unknown function.
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