The effect of the extracellular matrix on the detachment of human endothelial cells
The effect of the extracellular matrix on the detachment of human endothelial cells
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细胞外基质对人内皮细胞脱离的影响
DOI：
10.1002/jcp.1041210304
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发表时间：
1984
影响因子：
5.6
通讯作者：
V. Hatcher
中科院分区：
文献类型：
--
作者：
P. Gordon;Mindy A. Levitt;C. Jenkins;V. Hatcher
人脐静脉内皮细胞可以通过补充具有部分纯化的生长因子的培养基连续传代。使用胰蛋白酶、胶原酶或同型半胱氨酸检查早期和晚期传代内皮细胞的细胞基质脱离。在所有试验条件下，晚期传代细胞比早期传代细胞更快地分离。分离率取决于所用的特定试剂。蛋白酶介导的脱离最快，发生在几分钟内，而同型半胱氨酸诱导的脱离发生在几小时内。当分离的细胞被收集并在不存在分离剂的情况下重新铺板时，这些细胞重新附着扩散，并继续增殖。在早期或晚期传代细胞与明胶基质的粘附率中未观察到显著差异。当将早期或晚期传代内皮细胞接种在由相反细胞类型合成的基质上并生长至汇合时，蛋白酶介导的细胞脱离速率与基质来源的细胞类型相似。内皮细胞脱离的难易程度由细胞外基质的来源决定。检查早期和晚期传代细胞的细胞外基质显示存在的糖胺聚糖和硫酸化蛋白的量存在显著差异。这些研究证明了内皮细胞细胞外基质在蛋白酶介导的细胞脱离中的重要性。细胞脱离的速率由细胞外基质控制，而不被内皮细胞改变。
Human umbilical vein endothelial cells can be serially passaged by supplementing medium with a partially purified growth factor. Cell‐substratum detachment of early and late passage endothelial cells was examined using trypsin, collagenase, or homocysteine. Late‐passage cells detached more rapidly than early passage cells under all conditions tested. The rate of detachment was dependent upon the specific agent used. Protease‐mediated detachment was most rapid, occurring over minutes, in contrast to homocysteine‐induced detachment, which occurred over hours. When detached cells were collected and replated in the absence of the detaching agent, these cells reattached spread, and continued to proliferate. No significant difference was observed in the rate of adhesion of either early or late passage cells to a gelatin matrix. When early or late‐passage endothelial cells were plated and grown to confluence on a matrix synthesized by the opposite cell type, the rate of protease‐mediated cell detachment resembled the cell type from which the matrix was derived. The ease of endothelial cell detachment was determined by the origin of the extracellular matrix. Examination of the extracellular matrices from early and late passage cells revealed significant differences in the amounts of glycosaminoglycans and sulfated proteins present. These studies demonstrate the importance of the endothelial cell extracellular matrix in protease‐mediated cell detachment. The rate of cell detachment was controlled by the extracellular matrices and not altered by the endothelial cells.
负责细胞增殖但不负责细胞附着的基底膜成分失活。
影响因子：
3.7
作者：
Greenburg,G;Gospodarowicz,D
通讯作者：
Gospodarowicz,D