A Novel Enzyme Portfolio for Red Algal Polysaccharide Degradation in the Marine Bacterium Paraglaciecola hydrolytica S66(T) Encoded in a Sizeable Polysaccharide Utilization Locus.
A Novel Enzyme Portfolio for Red Algal Polysaccharide Degradation in the Marine Bacterium Paraglaciecola hydrolytica S66(T) Encoded in a Sizeable Polysaccharide Utilization Locus.
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DOI：
10.3389/fmicb.2018.00839
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发表时间：
2018
影响因子：
5.2
通讯作者：
Stougaard P
中科院分区：
文献类型：
--
作者：
Schultz-Johansen M;Bech PK;Hennessy RC;Glaring MA;Barbeyron T;Czjzek M;Stougaard P
海洋微生物是降解多种多糖酶的丰富来源。副水解菌S66T是一株能够降解红藻细胞壁中发现的多糖的海洋细菌。在这项研究中，我们应用了一种结合基因组挖掘和功能分析的方法来揭示该细菌产生酶的潜力，以用于复杂的海洋多糖的水解酶。水解菌S66T的一个特殊特征是存在一个大的基因组区域，含有一系列碳水化合物活性酶(CAZymes)，特别是琼脂酶和卡拉胶酶。在首次功能鉴定的基础上，结合比较序列分析，我们确定了该细菌降解红藻多糖所需的几种酶的活性。特别是，我们首次报道了针对呋喃的新型酶活性的发现，呋喃-卡拉胶是一种同时含有β-卡拉胶和κ/β-卡拉胶基序的杂交卡拉胶。其中一些酶代表了CAZy分类中的一个新的亚家族。通过综合分析，我们提出了水解菌S66T对琼脂和κ/β类卡拉胶的完全降解模型。本文描述的新型酶可能会在新的生物产业中发现价值，并促进我们对海洋生态系统中红藻多糖循环机制的理解。
Marine microbes are a rich source of enzymes for the degradation of diverse polysaccharides. Paraglaciecola hydrolytica S66T is a marine bacterium capable of hydrolyzing polysaccharides found in the cell wall of red macroalgae. In this study, we applied an approach combining genomic mining with functional analysis to uncover the potential of this bacterium to produce enzymes for the hydrolysis of complex marine polysaccharides. A special feature of P. hydrolytica S66T is the presence of a large genomic region harboring an array of carbohydrate-active enzymes (CAZymes) notably agarases and carrageenases. Based on a first functional characterization combined with a comparative sequence analysis, we confirmed the enzymatic activities of several enzymes required for red algal polysaccharide degradation by the bacterium. In particular, we report for the first time, the discovery of novel enzyme activities targeting furcellaran, a hybrid carrageenan containing both β-carrageenan and κ/β-carrageenan motifs. Some of these enzymes represent a new subfamily within the CAZy classification. From the combined analyses, we propose models for the complete degradation of agar and κ/β-type carrageenan by P. hydrolytica S66T. The novel enzymes described here may find value in new bio-based industries and advance our understanding of the mechanisms responsible for recycling of red algal polysaccharides in marine ecosystems.
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影响因子：
5
作者：
Kim, JH;Byun, DS;Kim, HR
通讯作者：
Kim, HR
用户融合：一种快速有效的方法，用于同时融合和克隆多个PCR产品。
影响因子：
14.9
作者：
Geu-Flores, Fernando;Nour-Eldin, Hussam H.;Nielsen, Morten T.;Halkier, Barbara A.
通讯作者：
Halkier, Barbara A.
影响因子：
3.7
作者：
Blanvillain S;Meyer D;Boulanger A;Lautier M;Guynet C;Denancé N;Vasse J;Lauber E;Arlat M
通讯作者：
Arlat M
影响因子：
4.8
作者：
Hehemann, Jan-Hendrik;Correc, Gaelle;Czjzek, Mirjam
通讯作者：
Czjzek, Mirjam
影响因子：
5.1
作者：
Bauer, Margarete;Kube, Michael;Gloeckner, Frank Oliver
通讯作者：
Gloeckner, Frank Oliver