All Staphylococcus aureus bacteraemia-inducing strains can cause infective endocarditis: Results of GWAS and experimental animal studies.
All Staphylococcus aureus bacteraemia-inducing strains can cause infective endocarditis: Results of GWAS and experimental animal studies.
复制标题
DOI：
10.1016/j.jinf.2022.12.028
复制
发表时间：
2023-02
影响因子：
28.2
通讯作者：
Vandenesch, Francois
中科院分区：
文献类型：
--
作者：
Bastien, Sylvere;Meyers, Severien;Salgado-Pabon, Wilmara;Giulieri, Stefano G.;Rasigade, Jean-Phillipe;Liesenborghs, Laurens;Kinney, Kyle J.;Couzon, Florence;Martins-Simoes, Patricia;Le Moing, Vincent;Duval, Xavier;Holmes, Natasha E.;Bruun, Niels Eske;Skov, Robert;Howden, Benjamin P.;Fowler, Vance G., Jr.;Verhamme, Peter;Andersen, Paal Skytt;Bouchiat, Coralie;Moreau, Karen;Vandenesch, Francois
关键词：
我们的目的是确定特异性金黄色葡萄球菌菌株是否在金黄色葡萄球菌菌血症（SAB）过程中引起感染性心内膜炎（IE）。研究人员进行了一项全基因组关联研究（GWAS），其中包括来自IE（274）和非IE (650) SAB患者的924个金黄色葡萄球菌基因组，并使用IE的两种实验动物模型对一部分菌株进行了测试，其中一种研究了细菌粘附炎症小鼠瓣膜的早期步骤，另一种评估了IE在机械损伤兔瓣膜上的局部和全身发育过程。考虑到GWAS分析的单核苷酸多态性、编码序列和k-mers，金黄色葡萄球菌IE和非IE SAB菌株的遗传谱没有差异。在小鼠炎症诱导的IE模型中，IE和非IE SAB菌株在粘附心脏瓣膜和引起IE的倾向方面没有差异；在机械IE诱导兔模型中，IE和非IE SAB菌株在植被大小和CFU方面没有差异。从SAB患者身上分离出的所有金黄色葡萄球菌菌株一旦进入血液，必须被认为能够引起这种常见和致命的感染。
We aimed at determining whether specific S. aureus strains cause infective endocarditis (IE) in the course of Staphylococcus aureus bacteraemia (SAB). A genome-wide association study (GWAS) including 924 S. aureus genomes from IE (274) and non-IE (650) SAB patients from international cohorts was conducted, and a subset of strains was tested with two experimental animal models of IE, one investigating the early step of bacterial adhesion to inflamed mice valves, the second evaluating the local and systemic developmental process of IE on mechanically-damaged rabbit valves. The genetic profile of S. aureus IE and non-IE SAB strains did not differ when considering single nucleotide polymorphisms, coding sequences, and k-mers analysed in GWAS. In the murine inflammation-induced IE model, no difference was observed between IE and non-IE SAB strains both in terms of adhesion to the cardiac valves and in the propensity to cause IE; in the mechanical IE-induced rabbit model, there was no difference between IE and non-IE SAB strains regarding the vegetation size and CFU. All strains of S. aureus isolated from SAB patients must be considered as capable of causing this common and lethal infection once they have accessed the bloodstream.
登录
查看更多内容
影响因子：
3.7
作者：
Holmes NE;Robinson JO;van Hal SJ;Munckhof WJ;Athan E;Korman TM;Cheng AC;Turnidge JD;Johnson PDR;Howden BP;VANESSA study group, on behalf of the Australasian Society for Infectious Diseases (ASID) Clinical Research Network (CRN)
通讯作者：
VANESSA study group, on behalf of the Australasian Society for Infectious Diseases (ASID) Clinical Research Network (CRN)
影响因子：
5
作者：
Cyr DD;Allen AS;Du GJ;Ruffin F;Adams C;Thaden JT;Maskarinec SA;Souli M;Guo S;Dykxhoorn DM;Scott WK;Fowler VG Jr
通讯作者：
Fowler VG Jr
影响因子：
5.8
作者：
Gu, Zuguang;Eils, Roland;Schlesner, Matthias
通讯作者：
Schlesner, Matthias
影响因子：
5.9
作者：
Crum, Nancy F.;Lee, Rachel U.;Russell, Kevin L.
通讯作者：
Russell, Kevin L.
影响因子：
2.1
作者：
Hill, PC;Birch, M;Morris, AJ
通讯作者：
Morris, AJ