Expression of fibronectin splice variants in the postischemic rat kidney.
Expression of fibronectin splice variants in the postischemic rat kidney.
复制标题
纤连蛋白剪接变体在缺血后大鼠肾脏中的表达。
DOI：
10.1152/ajprenal.2001.280.6.f1037
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Matlin,KS
中科院分区：
文献类型：
--
作者：
Zuk,A;Bonventre,JV;Matlin,KS
使用单侧肾缺血的体内大鼠模型，我们以前表明，纤维连接蛋白（FN），血浆和细胞外基质的主要糖蛋白的表达和分布，响应缺血再灌注显着变化。特别是在远端肾单位，FN积聚在管腔中，可能导致梗阻。在本研究中，我们检查是否管状FN是血浆或细胞形式，其中每一个是由一个单一的基因转录的选择性剪接产生的。我们证明，FN在管腔不包含额外的III型A（EIIIA）和/或额外的III型B（EIIIB）区域，这两者都是独特的细胞FN。然而，它确实含有V95区，在大鼠中，V95区是血浆和细胞外基质中FN的组分。缺血性损伤后，含FN的EIIIA在肾髓质中的表达显著增加，并在6周后继续以高水平产生。含V95的FN也在间质中增加，尽管比含FN的EIIIA更慢且水平更低; 6周后也持续存在。含有EIIIB区的FN在损伤的肾脏中不表达。肾小管中FN的V95而非EIIIA或EIIIB区的存在表明该位置的FN来源为血浆;肾小管FN最终在尿液中排出。这些数据表明，血浆和细胞FN含有V95和/或EIIIA区域可能有助于急性肾功能衰竭的发病机制和损伤的肾脏的修复。
Using an in vivo rat model of unilateral renal ischemia, we previously showed that the expression and distribution of fibronectin (FN), a major glycoprotein of plasma and the extracellular matrix, dramatically changes in response to ischemia-reperfusion. In the distal nephron in particular, FN accumulates in tubular lumens, where it may contribute to obstruction. In the present study, we examine whether the tubular FN is the plasma or cellular form, each of which is produced by alternative splicing of a single gene transcript. We demonstrate that FN in tubular lumens does not contain the extra type III A (EIIIA) and/or the extra type III B (EIIIB) region, both of which are unique to cellular FN. It does, however, contain the V95 region, which in the rat is a component of FNs in both plasma and the extracellular matrix. Expression of FN containing EIIIA increases dramatically in the renal interstitium after ischemic injury and continues to be produced at high levels 6 wk later. V95-containing FN also increases in the interstitial space, albeit more slowly and at lower levels than FN containing EIIIA; it also persists 6 wk later. FN containing the EIIIB region is not expressed in the injured kidney. The presence of V95 but not the EIIIA or EIIIB regions of FN in tubular lumens identifies the origin of FN in this location as the plasma; tubular FN is ultimately voided in the urine. The data indicate that both plasma and cellular FNs containing the V95 and/or EIIIA regions may contribute to the pathogenesis of acute renal failure and to the repair of the injured kidney.
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DOI：
--
发表时间：
1993-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Chuanyue Wu;J. Bauer;R. Juliano;John A. McDonald
通讯作者：
Chuanyue Wu;J. Bauer;R. Juliano;John A. McDonald
血浆纤连蛋白由肝细胞合成和分泌。
影响因子：
4.8
作者：
J. Tamkun;R. Hynes
通讯作者：
R. Hynes
DOI：
--
发表时间：
1986-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Ignotz;J. Massagué
通讯作者：
R. Ignotz;J. Massagué
DOI：
--
发表时间：
1977
期刊：
Virchows Archiv B Cell Pathology
影响因子：
--
作者：
B. Glaumann;H. Glaumann;B. Trump
通讯作者：
B. Trump
缺血性损伤后肾皮质的变化。
DOI：
--
发表时间：
1972
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
K. Reimer;C. Ganote;R. Jennings
通讯作者：
R. Jennings