Sequence Analysis of Tn10 Insertion Sites in a Collection of Escherichia coli Strains Used for Genetic Mapping and Strain Construction
Sequence Analysis of Tn10 Insertion Sites in a Collection of Escherichia coli Strains Used for Genetic Mapping and Strain Construction
复制标题
用于遗传作图和菌株构建的一组大肠杆菌菌株中 Tn10 插入位点的序列分析
DOI：
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发表时间：
1998
影响因子：
3.2
通讯作者：
Victoria Meiners
中科院分区：
文献类型：
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作者：
B. Nichols;Obaid Shafiq;Victoria Meiners
摘要利用反向PCR技术扩增了含Tn10的大肠杆菌染色体插入位点，并测定了其连接处的核苷酸序列。在95个分析菌株中，确定了88个独特的Tn10位点，并与E. coli染色体序列。在插入位点的位置上有两个缺口，一个包括DNA复制的末端，另一个由重组热点RhsA和RhsB界定。
ABSTRACT The chromosomal insertion sites of Tn10-containingEscherichia coli strains were amplified by inverse PCR, and the nucleotide sequences of the junctions were determined. In 95 strains analyzed, 88 unique Tn10 positions were determined and matched to the E. coli chromosome sequence. Two gaps in insertion site positions were noted, one including the terminus of DNA replication and another bounded by recombination hot spots RhsA and RhsB.
含有遗传连锁的交替抗生素抗性元件的菌株集合，用于大肠杆菌的遗传图谱。
DOI：
10.1128/mr.53.1.1-24.1989
发表时间：
1989
期刊：
Microbiological reviews
影响因子：
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作者：
Singer,M;Baker,TA;Schnitzler,G;Deischel,SM;Goel,M;Dove,W;Jaacks,KJ;Grossman,AD;Erickson,JW;Gross,CA
通讯作者：
Gross,CA
影响因子：
5.6
作者：
LIN, RJ;CAPAGE, M;HILL, CW
通讯作者：
HILL, CW