Identification of putative effectors of the Type IV secretion system from the Wolbachia endosymbiont of Brugia malayi.
Identification of putative effectors of the Type IV secretion system from the Wolbachia endosymbiont of Brugia malayi.
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DOI：
10.1371/journal.pone.0204736
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Starai VJ
中科院分区：
文献类型：
--
作者：
Carpinone EM;Li Z;Mills MK;Foltz C;Brannon ER;Carlow CKS;Starai VJ
沃尔巴克氏体是一种不可培养的细胞内细菌，存在于非常广泛的节肢动物和寄生线虫宿主中，通过垂直传播将其母体传播给后代。在马来丝虫（Brugia malayi）中，沃尔巴克氏体是一种内共生体，它的存在对于线虫的正常发育、存活和发病是必不可少的。虽然阐明促进细菌细胞内持久性的沃尔巴克氏体：线虫相互作用是非常重要的，但由于沃尔巴克氏体不能在没有宿主细胞的情况下培养的事实，研究受到阻碍。B的Wolbachia内共生体。马来人（wBm）具有活跃的IV型分泌系统（T4 SS）。在这里，我们已经筛选了47个假定的T4 SS效应蛋白的wBM的调节生长或细胞生物学的一个典型的真核细胞，酿酒酵母的能力。5个候选物在表达后强烈抑制酵母生长，并且6个另外的蛋白质在锌和咖啡因的存在下显示毒性。对内吞液泡特异性荧光标记物FM 4 -64的摄取的研究鉴定了参与液泡膜动力学的4种蛋白质（wBm 0076、wBm 00114、wBm 0447和wBm 0152）。发现WAS（p）家族蛋白wBm 0076与酵母皮质肌动蛋白斑块共定位，并在表达时破坏肌动蛋白细胞骨架动力学。Arp 2/3激活蛋白Abp 1 p的缺失对wBm 0076的表达具有抗性，表明wBm 0076在调节真核细胞肌动蛋白动力学和皮层肌动蛋白斑块形成中具有作用。此外，发现wBm 0152强烈破坏酵母中的内体：空泡货物运输。这项研究提供了分子洞察的潜在作用的T4 SS在沃尔巴克氏体内共生体：线虫的关系。
Wolbachia is an unculturable, intracellular bacterium that persists within an extremely broad range of arthropod and parasitic nematode hosts, where it is transmitted maternally to offspring via vertical transmission. In the filarial nematode Brugia malayi, a causative agent of human lymphatic filariasis, Wolbachia is an endosymbiont, and its presence is essential for proper nematode development, survival, and pathogenesis. While the elucidation of Wolbachia:nematode interactions that promote the bacterium’s intracellular persistence is of great importance, research has been hampered due to the fact that Wolbachia cannot be cultured in the absence of host cells. The Wolbachia endosymbiont of B. malayi (wBm) has an active Type IV secretion system (T4SS). Here, we have screened 47 putative T4SS effector proteins of wBm for their ability to modulate growth or the cell biology of a typical eukaryotic cell, Saccharomyces cerevisiae. Five candidates strongly inhibited yeast growth upon expression, and 6 additional proteins showed toxicity in the presence of zinc and caffeine. Studies on the uptake of an endocytic vacuole-specific fluorescent marker, FM4-64, identified 4 proteins (wBm0076 wBm00114, wBm0447 and wBm0152) involved in vacuole membrane dynamics. The WAS(p)-family protein, wBm0076, was found to colocalize with yeast cortical actin patches and disrupted actin cytoskeleton dynamics upon expression. Deletion of the Arp2/3-activating protein, Abp1p, provided resistance to wBm0076 expression, suggesting a role for wBm0076 in regulating eukaryotic actin dynamics and cortical actin patch formation. Furthermore, wBm0152 was found to strongly disrupt endosome:vacuole cargo trafficking in yeast. This study provides molecular insight into the potential role of the T4SS in the Wolbachia endosymbiont:nematode relationship.
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影响因子：
2.1
作者：
Aleman, Ainel;Fernandez-Pinar, Pablo;Molina, Maria
通讯作者：
Molina, Maria
芽殖酵母酿酒酵母中内体和肌动蛋白斑块的组装、拆卸和肌动蛋白电缆依赖性运动的活细胞成像。
影响因子：
7.8
作者：
Huckaba, TM;Gay, AC;Pantalena, LF;Yang, HC;Pon, LA
通讯作者：
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影响因子：
9.2
作者：
Bone, N;Millar, JBA;Armstrong, J
通讯作者：
Armstrong, J
影响因子：
6.4
作者：
Bennuru S;Cotton JA;Ribeiro JM;Grote A;Harsha B;Holroyd N;Mhashilkar A;Molina DM;Randall AZ;Shandling AD;Unnasch TR;Ghedin E;Berriman M;Lustigman S;Nutman TB
通讯作者：
Nutman TB
影响因子：
4
作者：
Gourlay, CW;Dewar, H;Ayscough, KR
通讯作者：
Ayscough, KR