Live cell imaging of the assembly, disassembly, and actin cable-dependent movement of endosomes and actin patches in the budding yeast, Saccharomyces cerevisiae.
Live cell imaging of the assembly, disassembly, and actin cable-dependent movement of endosomes and actin patches in the budding yeast, Saccharomyces cerevisiae.
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芽殖酵母酿酒酵母中内体和肌动蛋白斑块的组装、拆卸和肌动蛋白电缆依赖性运动的活细胞成像。
DOI：
10.1083/jcb.200404173
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发表时间：
2004-11-08
影响因子：
7.8
通讯作者：
Pon, LA
中科院分区：
文献类型：
--
作者：
Huckaba, TM;Gay, AC;Pantalena, LF;Yang, HC;Pon, LA
用Fm4-标记内小体，用Abp1p-GFP或Sac6P-GFP标记肌动蛋白斑块，我们发现：(1)内小体与肌动蛋白斑块在芽皮层组装时共定位；(2)内体与肌动蛋白斑块从芽向母细胞线性逆行运动时共定位；(3)肌动蛋白斑块与FM4-标记的内部隔室相互作用和分解。我们还表明，肌动蛋白电缆的逆行流动介导了肌动蛋白斑块的逆行运动。Arp2/3复合体突变降低了皮质、非线性肌动蛋白斑块运动的频率，但对线性、逆行肌动蛋白斑块运动的速度没有影响。相反，线性肌动蛋白斑块的移动速度和方向与肌动蛋白缆索的移动速度和方向相同。此外，肌动蛋白补片需要肌动蛋白电缆进行逆行运动，并在进行逆行运动时与肌动蛋白电缆共存。我们的研究支持一种机制，即肌动蛋白缆索作为“传送带”逆行移动并将肌动蛋白斑块/内小体运送到FM4-标记的内室。
Using FM4-64 to label endosomes and Abp1p-GFP or Sac6p-GFP to label actin patches, we find that (1) endosomes colocalize with actin patches as they assemble at the bud cortex; (2) endosomes colocalize with actin patches as they undergo linear, retrograde movement from buds toward mother cells; and (3) actin patches interact with and disassemble at FM4-64–labeled internal compartments. We also show that retrograde flow of actin cables mediates retrograde actin patch movement. An Arp2/3 complex mutation decreases the frequency of cortical, nonlinear actin patch movements, but has no effect on the velocity of linear, retrograde actin patch movement. Rather, linear actin patch movement occurs at the same velocity and direction as the movement of actin cables. Moreover, actin patches require actin cables for retrograde movements and colocalize with actin cables as they undergo retrograde movement. Our studies support a mechanism whereby actin cables serve as “conveyor belts” for retrograde movement and delivery of actin patches/endosomes to FM4-64–labeled internal compartments.
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影响因子：
64.8
作者：
Lappalainen, P;Drubin, DG
通讯作者：
Drubin, DG
影响因子：
21.3
作者：
Merrifield, CJ;Moss, SE;Almers, W
通讯作者：
Almers, W
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2.6
作者：
GIETZ, RD;SCHIESTL, RH;WOODS, RA
通讯作者：
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3.3
作者：
Krueger, EW;Orth, JD;McNiven, MA
通讯作者：
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21.3
作者：
Merrifield, CJ;Feldman, ME;Almers, W
通讯作者：
Almers, W