Evolutionary plasticity of restorer-of-fertility-like proteins in rice.

Evolutionary plasticity of restorer-of-fertility-like proteins in rice.
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DOI:
10.1038/srep35152
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发表时间:
2016-10-24
期刊:
影响因子:
4.6
通讯作者:
Small I
Small I
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Melonek J;Stone JD;Small I

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水稻杂交制种依赖于特定线粒体蛋白诱导的细胞质雄性不育(CMS),其有害作用被育性核恢复基因(RF)所抑制。大多数RF蛋白属于RNA结合的五肽重复蛋白家族中的一个特定分支。我们已经从13个水稻基因组和Brachypodium disachyon基因组中确定了‘育性恢复类似’(RFL)序列的特征。大多数RFL序列存在于位于两个或三个染色体位点的基因组簇中,只有一小部分作为分离基因存在。RFL基因组簇位于水稻10号染色体上,几乎所有已知的水稻活性RF基因都位于该染色体上,其结构和基因含量在物种间存在极大差异。我们证明了同源重组事件是在RFL蛋白质中产生大量RNA序列识别基序的有效机制,也是RFL序列进化的主要驱动力。这些RFL序列将加深我们对CMS和植物育性恢复的分子基础的理解,并将加速新育种策略的发展。
Hybrid seed production in rice relies on cytoplasmic male sterility (CMS) induced by specific mitochondrial proteins, whose deleterious effects are suppressed by nuclear Restorer of Fertility (RF) genes. The majority of RF proteins belong to a specific clade of the RNA-binding pentatricopeptide repeat protein family. We have characterised ‘restorer-of-fertility-like’ (RFL) sequences from 13 Oryza genomes and the Brachypodium distachyon genome. The majority of the RFL sequences are found in genomic clusters located at two or three chromosomal loci with only a minor proportion being present as isolated genes. The RFL genomic cluster located on Oryza chromosome 10, the location of almost all known active rice RF genes, shows extreme variation in structure and gene content between species. We show evidence for homologous recombination events as an efficient mechanism for generating the huge repertoire of RNA sequence recognition motifs within RFL proteins and a major driver of RFL sequence evolution. The RFL sequences identified here will improve our understanding of the molecular basis of CMS and fertility restoration in plants and will accelerate the development of new breeding strategies.
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