Identification of novel genes selectively expressed in the follicle-associated epithelium from the meta-analysis of transcriptomics data from multiple mouse cell and tissue populations.
Identification of novel genes selectively expressed in the follicle-associated epithelium from the meta-analysis of transcriptomics data from multiple mouse cell and tissue populations.
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DOI：
10.1093/dnares/dss022
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发表时间：
2012-10
期刊：
影响因子：
--
通讯作者：
Mabbott NA
中科院分区：
文献类型：
--
作者：
Kobayashi A;Donaldson DS;Kanaya T;Fukuda S;Baillie JK;Freeman TC;Ohno H;Williams IR;Mabbott NA
Peyer集合淋巴结上的滤泡相关上皮（FAE）及其内的微褶细胞（M细胞）是抗原跨肠上皮转胞吞的重要部位。使用荟萃分析的方法，我们确定了一个转录签名，区分FAE从大量的小鼠细胞和组织。共表达的21个FAE特异性基因的集群进行了鉴定，并在其启动子区的转录因子结合位点基序的分析表明，这些基因共享一个潜在的转录程序。该簇包含已知的FAE-（Anxa 10、Ccl 20、Psg 18和Ubd）和M-细胞特异性（Gp 2）基因，表明其他基因是新的FAE特异性基因。这些新的候选基因中的一些在FAE和M细胞中高度表达（Calcb、Ces 3b、Clca 2和Gjb 2），而另一些仅在FAE中高度表达（Ascl 2、Cftr、Fgf 15、Gpr 133、Kcna 1、Kcnj 15、Mycl 1、Pgap 1和Rps 6 kl）。我们还鉴定了核因子（NF）受体激活剂-κB配体刺激后在肠上皮中诱导的新型FAE相关基因的一个子集。这些包括对FAE肠细胞特异性的Mfge 8。这项研究为FAE转录组提供了新的见解。进一步表征这里确定的候选基因将有助于识别FAE中细胞功能的新调节因子。
The follicle-associated epithelium (FAE) overlying the Peyer's patches and the microfold cells (M cells) within it are important sites of antigen transcytosis across the intestinal epithelium. Using a meta-analysis approach, we identified a transcriptional signature that distinguished the FAE from a large collection of mouse cells and tissues. A co-expressed cluster of 21 FAE-specific genes was identified, and the analysis of the transcription factor binding site motifs in their promoter regions indicated that these genes shared an underlying transcriptional programme. This cluster contained known FAE- (Anxa10, Ccl20, Psg18 and Ubd) and M-cell-specific (Gp2) genes, suggesting that the others were novel FAE-specific genes. Some of these novel candidate genes were expressed highly by the FAE and M cells (Calcb, Ces3b, Clca2 and Gjb2), and others only by the FAE (Ascl2, Cftr, Fgf15, Gpr133, Kcna1, Kcnj15, Mycl1, Pgap1 and Rps6kl). We also identified a subset of novel FAE-related genes that were induced in the intestinal epithelium after receptor activator of nuclear factor (NF)-κB ligand stimulation. These included Mfge8 which was specific to FAE enterocytes. This study provides new insight into the FAE transcriptome. Further characterization of the candidate genes identified here will aid the identification of novel regulators of cell function in the FAE.
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影响因子：
56.9
作者：
Carninci, P;Kasukawa, T;Hayashizaki, Y
通讯作者：
Hayashizaki, Y
影响因子：
2.8
作者：
Bradford, Barry M.;Sester, David P.;Mabbott, Neil A.
通讯作者：
Mabbott, Neil A.
Sox17 促进细胞周期进程并抑制 TGF-β/Smad3 信号传导以启动呼吸道上皮中的祖细胞行为。
影响因子：
3.7
作者：
Lange AW;Keiser AR;Wells JM;Zorn AM;Whitsett JA
通讯作者：
Whitsett JA
影响因子：
5.3
作者：
de Lau, Wim;Kujala, Pekka;Clevers, Hans
通讯作者：
Clevers, Hans
影响因子：
4.6
作者：
D'Angelo, Anna;Bluteau, Olivier;Pontoglio, Marco
通讯作者：
Pontoglio, Marco