SARS-CoV-2 antibody magnitude and detectability are driven by disease severity, timing, and assay.
SARS-CoV-2 antibody magnitude and detectability are driven by disease severity, timing, and assay.
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DOI：
10.1126/sciadv.abh3409
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发表时间：
2021-07
期刊：
影响因子：
13.6
通讯作者：
Greenhouse B
中科院分区：
文献类型：
--
作者：
Peluso MJ;Takahashi S;Hakim J;Kelly JD;Torres L;Iyer NS;Turcios K;Janson O;Munter SE;Thanh C;Donatelli J;Nixon CC;Hoh R;Tai V;Fehrman EA;Hernandez Y;Spinelli MA;Gandhi M;Palafox MA;Vallari A;Rodgers MA;Prostko J;Hackett J Jr;Trinh L;Wrin T;Petropoulos CJ;Chiu CY;Norris PJ;DiGermanio C;Stone M;Busch MP;Elledge SK;Zhou XX;Wells JA;Shu A;Kurtz TW;Pak JE;Wu W;Burbelo PD;Cohen JI;Rutishauser RL;Martin JN;Deeks SG;Henrich TJ;Rodriguez-Barraquer I;Greenhouse B
先前 SARS-CoV-2 感染的抗体检测取决于严重程度、检测方法和时间，对血清监测有影响。严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 血清监测研究的解释受到不同临床表现个体中随着时间的推移抗体检测性能定义不明确的限制。我们使用 14 项检测方法测量了 160 天内 128 人血浆样本中的抗体反应。我们发现，在发烧、咳嗽、住院和需氧量的推动下，疾病严重程度对抗体量有一致而强烈的影响。对刺突蛋白与核衣壳的反应始终与中和具有较高的相关性。在恢复期早期和血清恢复时间期间，检测的灵敏度有很大差异。对于轻度感染的个体来说，变异性很大，他们的抗体滴度始终较低，商业化验的 6 个月灵敏度范围为 33% 至 98%。因此，检测 SARS-CoV-2 先前感染的能力在很大程度上取决于感染的严重程度、时间和所使用的检测方法。这些发现对 SARS-CoV-2 血清监测研究的设计和解释具有重要意义。
Antibody detection of prior SARS-CoV-2 infection depends on severity, assay, and timing with implications for serosurveillance. Interpretation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serosurveillance studies is limited by poorly defined performance of antibody assays over time in individuals with different clinical presentations. We measured antibody responses in plasma samples from 128 individuals over 160 days using 14 assays. We found a consistent and strong effect of disease severity on antibody magnitude, driven by fever, cough, hospitalization, and oxygen requirement. Responses to spike protein versus nucleocapsid had consistently higher correlation with neutralization. Assays varied substantially in sensitivity during early convalescence and time to seroreversion. Variability was dramatic for individuals with mild infection, who had consistently lower antibody titers, with sensitivities at 6 months ranging from 33 to 98% for commercial assays. Thus, the ability to detect previous infection by SARS-CoV-2 is highly dependent on infection severity, timing, and the assay used. These findings have important implications for the design and interpretation of SARS-CoV-2 serosurveillance studies.
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影响因子：
64.8
作者：
Gaebler C;Wang Z;Lorenzi JCC;Muecksch F;Finkin S;Tokuyama M;Cho A;Jankovic M;Schaefer-Babajew D;Oliveira TY;Cipolla M;Viant C;Barnes CO;Bram Y;Breton G;Hägglöf T;Mendoza P;Hurley A;Turroja M;Gordon K;Millard KG;Ramos V;Schmidt F;Weisblum Y;Jha D;Tankelevich M;Martinez-Delgado G;Yee J;Patel R;Dizon J;Unson-O'Brien C;Shimeliovich I;Robbiani DF;Zhao Z;Gazumyan A;Schwartz RE;Hatziioannou T;Bjorkman PJ;Mehandru S;Bieniasz PD;Caskey M;Nussenzweig MC
通讯作者：
Nussenzweig MC
影响因子：
2
作者：
Zhao, Jijun;Wang, Qin;Zhang, Jianhua
通讯作者：
Zhang, Jianhua
影响因子：
82.9
作者：
Long, Quan-Xin;Liu, Bai-Zhong;Huang, Ai-Long
通讯作者：
Huang, Ai-Long
DOI：
10.1016/s1473-3099(20)30634-4
发表时间：
2020-12
期刊：
The Lancet. Infectious diseases
影响因子：
--
作者：
National SARS-CoV-2 Serology Assay Evaluation Group
通讯作者：
National SARS-CoV-2 Serology Assay Evaluation Group
影响因子：
82.9
作者：
Amanat F;Stadlbauer D;Strohmeier S;Nguyen THO;Chromikova V;McMahon M;Jiang K;Arunkumar GA;Jurczyszak D;Polanco J;Bermudez-Gonzalez M;Kleiner G;Aydillo T;Miorin L;Fierer DS;Lugo LA;Kojic EM;Stoever J;Liu STH;Cunningham-Rundles C;Felgner PL;Moran T;García-Sastre A;Caplivski D;Cheng AC;Kedzierska K;Vapalahti O;Hepojoki JM;Simon V;Krammer F
通讯作者：
Krammer F