Pyrosequencing-based assessment of bacterial community structure along different management types in German forest and grassland soils.
Pyrosequencing-based assessment of bacterial community structure along different management types in German forest and grassland soils.
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DOI：
10.1371/journal.pone.0017000
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发表时间：
2011-02-16
期刊：
影响因子：
3.7
通讯作者：
Daniel R
中科院分区：
文献类型：
--
作者：
Nacke H;Thürmer A;Wollherr A;Will C;Hodac L;Herold N;Schöning I;Schrumpf M;Daniel R
土壤细菌是陆地生态系统中几乎所有生物地球化学循环的重要驱动因素，并参与土壤中的大多数养分转化。与土壤细菌对生态系统功能的重要性相比，我们对不同的管理类型如何影响土壤细菌群落组成知之甚少。我们使用基于焦磷酸测序的 V2-V3 16S rRNA 基因区域分析来识别施瓦本阿尔布 (Schwäbische Alb) 的九个森林和九个草原土壤中细菌多样性和群落结构的变化，这些土壤涵盖了六种不同的管理类型。该数据集包含 598,962 个属于细菌领域的序列。每个样本的分类序列数量范围为 23,515 至 39,259。草地土壤中的细菌多样性比森林土壤中的细菌门更丰富。所有样本中的主要分类群（>所有序列的 1%）是酸杆菌门、Alphaproteobacteria、放线菌门、Betaproteobacteria、Deltaproteobacteria、Gammaproteobacteria 和厚壁菌门。观察到土地利用类型森林和草地之间细菌门和变形菌门（包括放线菌门、厚壁菌门、疣微菌门、蓝细菌门、Gemmatimonadetes 和 Alphaproteobacteria）的相对丰度存在显着差异。在属水平上，苯杆菌属、芽孢杆菌属、克里贝拉属、链霉菌属、农杆菌属和德氟球菌属等优势属也存在显着差异。此外，山毛榉林和云杉林土壤的土壤细菌群落结构也存在显着差异。不同分类水平的细菌群的相对丰度与土壤 pH 值相关，但与管理类型和其他土壤特性关系不大或没有关系。六种分析管理类型的土壤细菌群落组成和多样性表明，土地利用类型草地和森林之间存在显着差异。此外，细菌群落结构很大程度上取决于树种和土壤 pH 值。
Soil bacteria are important drivers for nearly all biogeochemical cycles in terrestrial ecosystems and participate in most nutrient transformations in soil. In contrast to the importance of soil bacteria for ecosystem functioning, we understand little how different management types affect the soil bacterial community composition. We used pyrosequencing-based analysis of the V2-V3 16S rRNA gene region to identify changes in bacterial diversity and community structure in nine forest and nine grassland soils from the Schwäbische Alb that covered six different management types. The dataset comprised 598,962 sequences that were affiliated to the domain Bacteria. The number of classified sequences per sample ranged from 23,515 to 39,259. Bacterial diversity was more phylum rich in grassland soils than in forest soils. The dominant taxonomic groups across all samples (>1% of all sequences) were Acidobacteria, Alphaproteobacteria, Actinobacteria, Betaproteobacteria, Deltaproteobacteria, Gammaproteobacteria, and Firmicutes. Significant variations in relative abundances of bacterial phyla and proteobacterial classes, including Actinobacteria, Firmicutes, Verrucomicrobia, Cyanobacteria, Gemmatimonadetes and Alphaproteobacteria, between the land use types forest and grassland were observed. At the genus level, significant differences were also recorded for the dominant genera Phenylobacter, Bacillus, Kribbella, Streptomyces, Agromyces, and Defluviicoccus. In addition, soil bacterial community structure showed significant differences between beech and spruce forest soils. The relative abundances of bacterial groups at different taxonomic levels correlated with soil pH, but little or no relationships to management type and other soil properties were found. Soil bacterial community composition and diversity of the six analyzed management types showed significant differences between the land use types grassland and forest. Furthermore, bacterial community structure was largely driven by tree species and soil pH.
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影响因子：
12.3
作者：
Huse SM;Huber JA;Morrison HG;Sogin ML;Welch DM
通讯作者：
Welch DM
DOI：
10.1073/pnas.0808254105
发表时间：
2008-11-18
影响因子：
11.1
作者：
Hartman, Wyatt H.;Richardson, Curtis J.;Bruland, Gregory L.
通讯作者：
Bruland, Gregory L.
DOI：
10.1099/00207713-50-2-633
发表时间：
2000-03-01
影响因子：
2.8
作者：
Anderson, RC;Rasmussen, MA;Allison, MJ
通讯作者：
Allison, MJ
影响因子：
9.7
作者：
Bååth, E;Anderson, TH
通讯作者：
Anderson, TH
影响因子：
3.3
作者：
Carmen Collado, Maria;Sanz, Yolanda
通讯作者：
Sanz, Yolanda