A high mitochondrial transport rate characterizes CNS neurons with high axonal regeneration capacity.
A high mitochondrial transport rate characterizes CNS neurons with high axonal regeneration capacity.
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DOI：
10.1371/journal.pone.0184672
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
He Z
中科院分区：
文献类型：
--
作者：
Cartoni R;Pekkurnaz G;Wang C;Schwarz TL;He Z
改善受损和患病的中枢神经系统中的轴突运输已被提出作为改善神经元修复的有希望的策略。然而，每种货物对修复机制的贡献是未知的。DRG神经元在再生过程中全面增加轴突运输。由于轴突损伤后特定货物的运输尚未在增强再生能力的模型中进行系统研究，因此尚不清楚所有货物的运输是否会在受损的中枢神经系统神经元中受到同等的调节。在这里，使用微流体培养系统，我们比较了共同缺失PTEN和SOCS 3的神经元，这是一种建立的高轴突再生能力模型，以控制神经元。我们测量了再生轴突中三种货物（线粒体、突触囊泡和晚期内体）的轴突运输，发现相对于对照，在PTEN/SOCS 3共缺失的轴突中线粒体的运输增加，而其他货物没有增加。这里报道的结果表明，轴突再生过程中，这个细胞器的关键作用。
Improving axonal transport in the injured and diseased central nervous system has been proposed as a promising strategy to improve neuronal repair. However, the contribution of each cargo to the repair mechanism is unknown. DRG neurons globally increase axonal transport during regeneration. Because the transport of specific cargos after axonal insult has not been examined systematically in a model of enhanced regenerative capacity, it is unknown whether the transport of all cargos would be modulated equally in injured central nervous system neurons. Here, using a microfluidic culture system we compared neurons co-deleted for PTEN and SOCS3, an established model of high axonal regeneration capacity, to control neurons. We measured the axonal transport of three cargos (mitochondria, synaptic vesicles and late endosomes) in regenerating axons and found that the transport of mitochondria, but not the other cargos, was increased in PTEN/SOCS3 co-deleted axons relative to controls. The results reported here suggest a pivotal role for this organelle during axonal regeneration.
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影响因子：
64.5
作者：
Pekkurnaz G;Trinidad JC;Wang X;Kong D;Schwarz TL
通讯作者：
Schwarz TL
影响因子：
16.2
作者：
Smith, Patrice D.;Sun, Fang;Park, Kevin Kyungsuk;Cai, Bin;Wang, Chen;Kuwako, Kenichiro;Martinez-Carrasco, Irene;Connolly, Lauren;He, Zhigang
通讯作者：
He, Zhigang
DOI：
10.1083/jcb.201605101
发表时间：
2016-07-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Zhou B;Yu P;Lin MY;Sun T;Chen Y;Sheng ZH
通讯作者：
Sheng ZH
影响因子：
16.2
作者：
Nawabi, Homaira;Belin, Stephane;Cartoni, Romain;Williams, Philip R.;Wang, Chen;Latremoliere, Alban;Wang, Xuhua;Zhu, Junjie;Taub, Daniel G.;Fu, Xiaoqin;Yu, Bin;Gu, Xiaosong;Woolf, Clifford J.;Liu, Judy S.;Gabel, Christopher V.;Steen, Judith A.;He, Zhigang
通讯作者：
He, Zhigang
影响因子：
16.2
作者：
Cartoni R;Norsworthy MW;Bei F;Wang C;Li S;Zhang Y;Gabel CV;Schwarz TL;He Z
通讯作者：
He Z