O-GlcNAc profiling: from proteins to proteomes.
O-GlcNAc profiling: from proteins to proteomes.
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DOI：
10.1186/1559-0275-11-8
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发表时间：
2014-03-05
影响因子：
3.8
通讯作者：
Hart GW
中科院分区：
文献类型：
--
作者：
Ma J;Hart GW
O-连接的β-D-N-乙酰葡萄糖胺（O-GlcNAc）修饰（O-GlcNAc化）是一种重要的蛋白质翻译后修饰（PTM），参与转录、翻译、蛋白酶体降解和信号转导等重要的生物学过程。异常蛋白质O-GlcNAc化与慢性疾病的病理进展直接相关，包括糖尿病、癌症和神经退行性疾病。O-GlcNAc蛋白的鉴定、位点定位和定量是破译其功能的先决条件。在这篇综述中，我们主要集中在技术的发展有关O-GlcNAc蛋白质谱。具体来说，一方面，我们展示了这些技术是如何被用于生物学家最感兴趣的某些目标蛋白质的综合表征。另一方面，我们提出了几种新开发的方法O-GlcNAcomic分析，以及他们如何为我们提供一个系统的角度，不同的PTM和复杂的生物事件之间的串扰。还强调了有前途的技术趋势，以唤起不同实验室的更多努力，这将进一步扩大我们对蛋白质O-GlcNAc酰化在慢性疾病中的生理和病理作用的理解。
O-linked β-D-N-acetylglucosamine (O-GlcNAc) modification (O-GlcNAcylation) onto serine and threonine residues of proteins is an important post-translational modification (PTM), which is involved in many crucial biological processes including transcription, translation, proteasomal degradation, and signal transduction. Aberrant protein O-GlcNAcylation is directly linked to the pathological progression of chronic diseases including diabetes, cancer, and neurodegenerative disorders. Identification, site mapping, and quantification of O-GlcNAc proteins are a prerequisite to decipher their functions. In this review, we mainly focus on technological developments regarding O-GlcNAc protein profiling. Specifically, on one hand, we show how these techniques are being used for the comprehensive characterization of certain targeted proteins in which biologists are most interested. On the other hand, we present several newly developed approaches for O-GlcNAcomic profiling as well as how they provide us with a systems perspective to crosstalk amongst different PTMs and complicated biological events. Promising technical trends are also highlighted to evoke more efforts by diverse laboratories, which would further expand our understanding of the physiological and pathological roles of protein O-GlcNAcylation in chronic diseases.
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影响因子：
3.2
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