Studies of bacterial aerotaxis in a microfluidic device.
Studies of bacterial aerotaxis in a microfluidic device.
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DOI：
10.1039/c2lc21006a
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发表时间：
2012-11-21
期刊：
影响因子：
6.1
通讯作者：
Groisman A
中科院分区：
文献类型：
--
作者：
Adler M;Erickstad M;Gutierrez E;Groisman A
趋氧性是细菌在氧气梯度中的定向运动，在世纪后期被发现，并且此后在多种细菌物种中被报道。然而，趋氧性的定量研究由于缺乏产生稳定的氧浓度梯度[O2]的工具而变得复杂。在这里，我们报告了一系列的实验，大肠杆菌在一个专门建造的实验装置，包括一个计算机控制的气体混合器和一个两层的聚二甲基硅氧烷（PDMS）制成的微流控装置的趋氧性。该装置能够在长梯度通道中生成各种稳定的[O2]线性曲线，其特征[O2]范围从有氧到微氧条件。E.大肠杆菌细胞以低速灌注通过梯度通道，使细胞有足够的时间探索[O2]梯度，并在通道出口附近以>105个细胞/小时的通量分析细胞在通道中的分布。在具有相同对数斜率和不同平均浓度的[O2]梯度中以及在具有相同平均浓度和不同斜率的梯度中进行趋氧性实验。在[O_2]浓度为0 ~11.5%的梯度中的实验表明，与以前的一些报道相反，E.大肠杆菌细胞不聚集在一些中等水平的[O2]，而是更喜欢最高的[O2]。该技术可应用于其他好氧和微好氧细菌的好氧性研究。
Aerotaxis, the directional motion of bacteria in gradients of oxygen, was discovered in late 19th century and has since been reported in a variety of bacterial species. Nevertheless, quantitative studies of aerotaxis have been complicated by the lack of tools for generation of stable gradients of oxygen concentration, [O2]. Here we report a series of experiments on aerotaxis of Escherichia coli in a specially built experimental setup consisting of a computer-controlled gas mixer and a two-layer microfluidic device made of polydimethylsiloxane (PDMS). The setup enables generation of a variety of stable linear profiles of [O2] across a long gradient channel, with characteristic [O2] ranging from aerobic to microaerobic conditions. A suspension of E. coli cells is perfused through the gradient channel at a low speed, allowing cells enough time to explore the [O2] gradient, and the distribution of cells across the channel is analyzed near the channel outlet at a throughput of >105 cells per hour. Aerotaxis experiments are performed in [O2] gradients with identical logarithmic slopes and varying mean concentrations, as well as in gradients with identical mean concentrations and varying slopes. Experiments in gradients with [O2] ranging from 0 to ~11.5% indicate that, in contrast to some previous reports, E. coli cells do not congregate at some intermediate level of [O2], but rather prefer the highest accessible [O2]. The presented technology can be applied to studies of aerotaxis of other aerobic and microaerobic bacteria.
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影响因子：
6.1
作者：
Adler M;Polinkovsky M;Gutierrez E;Groisman A
通讯作者：
Groisman A
影响因子：
5.1
作者：
Eschemann, A;Kühl, M;Cypionka, H
通讯作者：
Cypionka, H
DOI：
10.1002/path.1700770228
发表时间：
1959-01-01
期刊：
JOURNAL OF PATHOLOGY AND BACTERIOLOGY
影响因子：
--
作者：
BARACCHINI, O;SHERRIS, JC
通讯作者：
SHERRIS, JC
DOI：
10.1073/pnas.0707122105
发表时间：
2007-12-18
影响因子：
11.1
作者：
Higgins, J. M.;Eddington, D. T.;Mahadevan, L.
通讯作者：
Mahadevan, L.
影响因子：
3.2
作者：
Alexandre, G;Greer, SE;Zhulin, IB
通讯作者：
Zhulin, IB