Intra-Articular Injections of Polyphenols Protect Articular Cartilage from Inflammation-Induced Degradation: Suggesting a Potential Role in Cartilage Therapeutics.
Intra-Articular Injections of Polyphenols Protect Articular Cartilage from Inflammation-Induced Degradation: Suggesting a Potential Role in Cartilage Therapeutics.
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DOI：
10.1371/journal.pone.0127165
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Tiku ML
中科院分区：
文献类型：
--
作者：
Natarajan V;Madhan B;Tiku ML
关节炎疾病，如骨关节炎和类风湿性关节炎，给社会造成了巨大的医疗负担。骨关节炎是一种在老年人中高发的退行性关节疾病，类风湿关节炎是一种自身免疫性炎症疾病，两者都会导致关节软骨不可逆的结构和功能损伤。本研究的目的是研究儿茶素、槲皮素、表没食子儿茶素没食子酸酯和单宁酸等多酚类物质对II型胶原交联的影响，以及这些物质在体内关节软骨降解中的作用。对多酚处理后的牛关节软骨外植体的热、酶和物理稳定性进行了评估。表没食子儿茶素没食子酸酯和单宁酸处理的外植体的热稳定性比天然软骨提高了约12℃，从而证实了软骨交联。多酚处理的软骨也显示出胶原降解百分比的显著降低和对胶原酶消化的糖胺聚糖的释放，表明多酚交联软骨对酶消化的物理完整性和抵抗力增加。在大鼠胶原性关节炎诱导前（预防）和诱导后（治疗）关节内注射多酚，观察其对软骨的保护作用。对后爪软骨损伤进行体积和组织形态学评分。关节内注射表没食子儿茶素没食子酸酯和单宁酸对关节炎症的发作时间和强度无显著影响。然而，关节软骨的组织形态学评分显示，预防组和治疗组的软骨降解显著减少，这表明关节内注射多酚与关节软骨结合，使其在持续炎症的情况下抵抗降解。这些研究确立了关节内注射多酚在稳定软骨胶原蛋白防止降解方面的价值，并表明可注射多酚在关节炎条件下保护软骨的独特有益作用。
Arthritic diseases, such as osteoarthritis and rheumatoid arthritis, inflict an enormous health care burden on society. Osteoarthritis, a degenerative joint disease with high prevalence among older people, and rheumatoid arthritis, an autoimmune inflammatory disease, both lead to irreversible structural and functional damage to articular cartilage. The aim of this study was to investigate the effect of polyphenols such as catechin, quercetin, epigallocatechin gallate, and tannic acid, on crosslinking type II collagen and the roles of these agents in managing in vivo articular cartilage degradation. The thermal, enzymatic, and physical stability of bovine articular cartilage explants following polyphenolic treatment were assessed for efficiency. Epigallocatechin gallate and tannic acid-treated explants showed >12 °C increase over native cartilage in thermal stability, thereby confirming cartilage crosslinking. Polyphenol-treated cartilage also showed a significant reduction in the percentage of collagen degradation and the release of glycosaminoglycans against collagenase digestion, indicating the increase physical integrity and resistance of polyphenol crosslinked cartilage to enzymatic digestion. To examine the in vivo cartilage protective effects, polyphenols were injected intra-articularly before (prophylactic) and after (therapeutic) the induction of collagen-induced arthritis in rats. The hind paw volume and histomorphological scoring was done for cartilage damage. The intra-articular injection of epigallocatechin gallate and tannic acid did not significantly influence the time of onset or the intensity of joint inflammation. However, histomorphological scoring of the articular cartilage showed a significant reduction in cartilage degradation in prophylactic- and therapeutic-groups, indicating that intra-articular injections of polyphenols bind to articular cartilage and making it resistant to degradation despite ongoing inflammation. These studies establish the value of intra-articular injections of polyphenol in stabilization of cartilage collagen against degradation and indicate the unique beneficial role of injectable polyphenols in protecting the cartilage in arthritic conditions.
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影响因子：
3.3
作者：
Sophia Fox AJ;Bedi A;Rodeo SA
通讯作者：
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DOI：
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